The use of a one-step PCR method for the identification of Microsporum canis and Trichophyton mentagrophytes infection of pets

Iwona Dąbrowska; Bożena Dworecka-Kaszak; Anna Brillowska-Dąbrowska

doi:10.18388/abp.2014_1909

The use of a one-step PCR method for the identification of Microsporum canis and Trichophyton mentagrophytes infection of pets

Abstract

Introduction: Dermatophytes are a closely related group of keratinophilic fungi. They encompass important etiological agents of superficial fungal infections. These fungi are able to invade keratinized tissues of humans and animals, causing dermatophytosis (ringworm) of hair, nails or skin. The aim: Traditional diagnostics of ringworm is based on morphological identification of cultured fungi and is time-consuming. Materials and methods: In this study, we applied a method patented by Brillowska-Dabrowska and coworkers (Brillowska- Dąbrowska A, Saunte DM, Arenderup MC, 2007, Fivehour diagnosis of dermatophyte nail infections with specific detection of Trichophyton rubrum. J Clin Microbiol 45: 1200–1204) which involves extraction of fungal DNA and PCR amplification with pan-dermatophyte primers to confirm the presence of dermatophytes. Results: The method used here is able to confirm the presence of dermatophytes DNA in pure cultures in less than 5 hours.

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Authors (3)

Iwona Dąbrowska
- SGGW Zakład Mikrobiologii, Katedra Nauk Przedklinicznych
Bożena Dworecka-Kaszak
- SGGW Zakład Mikrobiologii, Katedra Nauk Przedklinicznych
Anna Brillowska-Dąbrowska dr hab. inż.

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Keywords

DERMATOPHYTOSIS, ANIMALS, PAN-DERMATOPHYTES, PCR

Details

Category:: Articles
Type:: artykuł w czasopiśmie wyróżnionym w JCR
Published in:: Acta Biochimica Polonica no. 61, pages 375 - 378,
ISSN: 0001-527X
Language:: English
Publication year:: 2014
Bibliographic description:: Dąbrowska I., Dworecka-Kaszak B., Brillowska-Dąbrowska A.: The use of a one-step PCR method for the identification of Microsporum canis and Trichophyton mentagrophytes infection of pets// Acta Biochimica Polonica. -Vol. 61, iss. 2 (2014), s.375-378
DOI:: Digital Object Identifier (open in new tab) 10.18388/abp.2014_1909
Verified by:: Gdańsk University of Technology