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• ALIS-FLP: amplified ligation selected fragment-length polymorphism method for microbial genotyping

  Publication

  • A. Brillowska-Dąbrowska
  • M. Wianecka
  • S. Dąbrowski
  • Z. Mladenovska
  • J. Kur
  • B. Ahring

  - SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION - Year 2008

  A DNA fingerprinting method known as ALIS-FLP (amplified ligation selected fragment-length polymorphism) has been developed for selective and specific amplification of restriction fragments from TspRI restriction endonuclease digested genomic DNA. The method is similar to AFLP, but differs in that only one specific restriction enzyme (TspRI) is used. The cohesive ends of the DNA fragments are ligated with two types of oligonucleotide....

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