
dr hab. Gracjana Klein-Raina
Employment
- Profesor uczelni at Laboratory Of Bacterial Genetics
Social media
Contact
- gracjana.klein@pg.edu.pl
Profesor uczelni
- Workplace
- Budynek B Wydziału Chemicznego pokój 04
- Phone
- (58) 347 26 18
Publication showcase
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Assembly of Lipopolysaccharide in Escherichia coli Requires the Essential LapB Heat Shock Protein
Here, we describe two new heat shock proteins involved in the assembly of LPS in Escherichia coli, LapA and LapB (lipopolysaccharide assembly protein A and B). lapB mutants were identified based on an increased envelope stress response. Envelope stress responsive pathways control key steps in LPS biogenesis and respond to defects in the LPS assembly. Accordingly, the LPS content in ΔlapB or Δ(lapA lapB) mutants was elevated, with...
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Regulated Assembly of LPS, Its Structural Alterations and Cellular Response to LPS Defects
Distinguishing feature of the outer membrane (OM) of Gram-negative bacteria is its asymmetry due to the presence of lipopolysaccharide (LPS) in the outer leaflet of the OM and phospholipids in the inner leaflet. Recent studies have revealed the existence of regulatory controls that ensure a balanced biosynthesis of LPS and phospholipids, both of which are essential for bacterial viability. LPS provides the essential permeability...
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Escherichia coli K-12 suppressor-free mutants lacking early glycosyltransferases and late acyltransferases. Minimal lipopolysaccharide structure and induction of envelope stress response.
To elucidate the minimal lipopolysaccharide (LPS) structure needed for the viability of Escherichia coli, suppressor-free strains lacking either the 3-deoxy-d-manno-oct-2-ulosonic acid transferase waaA gene or derivatives of the heptosyltransferase I waaC deletion with lack of one or all late acyltransferases (lpxL/M/P) and/or various outer membrane biogenesis factors were constructed. Δ(waaC lpxL lpxM lpxP) and waaA mutants exhibited...
Obtained scientific degrees/titles
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2017-09-26
Obtained science degree
dr hab. Microbiology (Biological sciences)Wydział Biologii i Ochrony Środowiska, Uniwersytet Łódzki -
1998-07-02
Obtained science degree
drWydzial Biologii, Geografii i Oceanologii, Uniwersytet Gdanski
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