Year 2010

• Characterization of exceptionally thermostable single-stranded DNA-binding proteins from Thermotoga maritima and Thermotoga neapolitana

  Publication

  • M. Olszewski
  • A. Grot
  • M. Wojciechowski
  • M. Nowak
  • M. Mickiewicz
  • J. Kur

  - BMC MICROBIOLOGY - Year 2010

  BACKGROUND: In recent years, there has been an increasing interest in SSBs because they find numerous applications in diverse molecular biology and analytical methods.RESULTS: We report the characterization of single-stranded DNA binding proteins (SSBs) from the thermophilic bacteria Thermotoga maritima (TmaSSB) and Thermotoga neapolitana (TneSSB). They are the smallest known bacterial SSB proteins, consisting of 141 and 142 amino...

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Year 2008

• Two highly thermostable paralogous single-stranded DNA-binding proteins from Thermoanaerobacter tengcongensis

  Publication

  • M. Olszewski
  • M. Mickiewicz
  • J. Kur

  - ARCHIVES OF MICROBIOLOGY - Year 2008

  The thermophilic bacterium Thermoanaerobacter tengcongensis has two single-stranded DNA-binding (SSB) proteins, designated TteSSB2 and TteSSB3. In a SSB complementation assay in Escherichia coli, only TteSSB3 took over the in vivo function of EcoSSB. We have cloned the ssb genes obtained by PCR and have developed E. coli overexpression systems. The TteSSB2 and TteSSB3 consist of 153 and 150 amino acids with a calculated molecular...

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