IKE1-IKE3 (C-1305 derivatives) inhibitory effect of the Yeast Topoisomerase II relaxation activity - Open Research Data - Bridge of Knowledge

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IKE1-IKE3 (C-1305 derivatives) inhibitory effect of the Yeast Topoisomerase II relaxation activity

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Description

Inhibition of Yeast Topoisomerase II were analyzed according to relaxation assay kit  from Inspiralis. Briefly, 250 ng of supercoiled pBR322 DNA, 1 mM ATP, 1-200 μM of analyzed compound were mixed with reaction buffer (1 mM Tris-HCl (pH 7.9), 10 mM KCl, 0.5 mM MgCl 2, 0.2 % (v/v) glycerol). The reaction was initiated by the addition of an enzyme, allowed to proceed at 30°C for 30 min and terminated by addition of 40% (w/v) sucrose, 100 mM Tris-HCl pH 8, 10 mM EDTA, 0.5 mg mL -1 Bromophenol Blue. Two step extraction with chloroform:isoamyl alcohol (24:1) and butanol water were made and mixtures were analyzed on the 1% agarose gel in 1x TAE buffer, 3h, 4.5 V cm -1. Gel was  stained in GelRed 3x staining solution for 30 min and photographed with Gel Doc XR+Gel Documentation System. 

Dataset file

130921 Yeast Topoisomerase II relaxation assay results, IKE1-IKE3.png
2.2 MB, S3 ETag 5468cadd3551707952cae84b69f03381-1, downloads: 20
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License:
Creative Commons: by-nc-sa 4.0 open in new tab
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Details

Year of publication:
2021
Verification date:
2021-11-18
Dataset language:
English
Fields of science:
  • Chemical sciences (Natural sciences)
DOI:
DOI ID 10.34808/d03p-7855 open in new tab
Funding:
Series:
Verified by:
Gdańsk University of Technology

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Version this document has several versions

DOI 10.34808/q2bm-7m23 represents the latest version of the data.

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