IKE1-IKE3 (C-1305 derivatives) inhibitory effect of the Yeast Topoisomerase II relaxation activity - Open Research Data - MOST Wiedzy

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IKE1-IKE3 (C-1305 derivatives) inhibitory effect of the Yeast Topoisomerase II relaxation activity

Description

Inhibition of Yeast Topoisomerase II were analyzed according to relaxation assay kit  from Inspiralis. Briefly, 250 ng of supercoiled pBR322 DNA, 1 mM ATP, 1-200 μM of analyzed compound were mixed with reaction buffer (1 mM Tris-HCl (pH 7.9), 10 mM KCl, 0.5 mM MgCl 2, 0.2 % (v/v) glycerol). The reaction was initiated by the addition of an enzyme, allowed to proceed at 30°C for 30 min and terminated by addition of 40% (w/v) sucrose, 100 mM Tris-HCl pH 8, 10 mM EDTA, 0.5 mg mL -1 Bromophenol Blue. Two step extraction with chloroform:isoamyl alcohol (24:1) and butanol water were made and mixtures were analyzed on the 1% agarose gel in 1x TAE buffer, 3h, 4.5 V cm -1. Gel was  stained in GelRed 3x staining solution for 30 min and photographed with Gel Doc XR+Gel Documentation System. 

Dataset file

130921 Yeast Topoisomerase II relaxation assay results, IKE1-IKE3.png
2.2 MB, S3 ETag 5468cadd3551707952cae84b69f03381-1, downloads: 4
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File details

License:
Creative Commons: CC BY-NC-SA 4.0 open in new tab
CC BY-NC-SA
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Details

Year of publication:
2021
Verification date:
2021-11-18
Dataset language:
English
Fields of science:
  • Chemical sciences (Natural sciences)
DOI:
DOI ID 10.34808/d03p-7855 open in new tab
Funding:
Series:
Verified by:
Gdańsk University of Technology

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