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IKE1-IKE3 (C-1305 derivatives) inhibitory effect of the Yeast Topoisomerase II relaxation activity
Description
Inhibition of Yeast Topoisomerase II were analyzed according to relaxation assay kit from Inspiralis. Briefly, 250 ng of supercoiled pBR322 DNA, 1 mM ATP, 1-200 μM of analyzed compound were mixed with reaction buffer (1 mM Tris-HCl (pH 7.9), 10 mM KCl, 0.5 mM MgCl 2, 0.2 % (v/v) glycerol). The reaction was initiated by the addition of an enzyme, allowed to proceed at 30°C for 30 min and terminated by addition of 40% (w/v) sucrose, 100 mM Tris-HCl pH 8, 10 mM EDTA, 0.5 mg mL -1 Bromophenol Blue. Two step extraction with chloroform:isoamyl alcohol (24:1) and butanol water were made and mixtures were analyzed on the 1% agarose gel in 1x TAE buffer, 3h, 4.5 V cm -1. Gel was stained in GelRed 3x staining solution for 30 min and photographed with Gel Doc XR+Gel Documentation System.
Dataset file
hexmd5(md5(part1)+md5(part2)+...)-{parts_count} where a single part of the file is 512 MB in size.Example script for calculation:
https://github.com/antespi/s3md5
File details
- License:
-
open in new tab
CC BY-NC-SANon-commercial - Share-alike - Raw data:
- Data contained in dataset was not processed.
Details
- Year of publication:
- 2021
- Verification date:
- 2021-11-18
- Dataset language:
- English
- Fields of science:
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- chemical sciences (Natural sciences)
- DOI:
- DOI ID 10.34808/d03p-7855 open in new tab
- Funding:
- Series:
- Verified by:
- Gdańsk University of Technology
Keywords
References
- dataset The absorption and fluorescence spectra of C-1305 derivatives (IKE1-IKE8), potential antifungal agents
- dataset Determination of the minimum inhibitory concentration of C-1305 derivatives (IKE1-IKE8) against Candida strains
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Authors
Version this document has several versions
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version 2.0release date 2022-01-20
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Current versionversion 1.0release date 2021-11-18
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