Search
Development of Safirinium dyes for new applications: fluorescent staining of bacteria, human kidney cells, and the horny layer of the epidermis
Abstract
Low-molecular synthetic fluorophores are convenient tools in bioimaging applications. Several derivatives of Safirinium dyes as well as their reactive N-hydroxysuccinimide (NHS) esters bearing diverse substituents were synthesized and evaluated experimentally in terms of their lipophilicity by means of reverse-phase and immobilized artificial membrane high-performance liquid chromatography. Subsequently, the selected compounds were employed as novel cellular imaging agents for staining Gram-positive and Gram-negative bacteria, human kidney cell line, as well as human skin tissue. The analyzed dyes allowed for visualization of cellular structures such as mitochondria, endoplasmic reticulum, and cellular nuclei. They proved to be useful in fluorescent staining of stratum corneum, especially in the aspect of xenobiotic exposure and its penetration into the skin. The best results were obtained with the use of moderately lipophilic NHS esters of Safirinium Q. The development of Safirinium dyes is a promising alternative for commercially available dyes since the reported molecules have low molecular masses and exhibit efficient staining and remarkable water solubility. Moreover, they are relatively simple and low-cost in synthesis.
Citations
-
2
CrossRef
-
0
Web of Science
-
2
Scopus
Authors (10)
-
Joanna Fedorowicz
- Drug Research Program, Division of Pharmaceutical Biosciences, Faculty of Pharmacy, University of Helsinki, P.O. Box 56 (ViikinkaarDepartment of Chemical Technology of Drugs, Faculty of Pharmac
-
Dagmara Bazar
- Department of Physical Chemistry, Medical University of Gdańsk, Al. Gen. Hallera 107, 80-416, Gdańsk, Poland
-
Hanna Kapica
- Department of Physical Chemistry, Medical University of Gdańsk, Al. Gen. Hallera 107, 80-416, Gdańsk, Poland.
-
Krzesimir Ciura
- Department of Physical Chemistry, Medical University of Gdańsk, Al. Gen. Hallera 107, 80-416, Gdańsk, Poland, QSAR Lab Ltd., Trzy Lipy 3 St., 80-172, Gdańsk, Poland
-
Krzysztof Cal
- Department of Pharmaceutical Technology, Medical University of Gdańsk, Al. Gen. J. Hallera 107, 80-416, Gdańsk, Poland
-
Krystyna Mojsiewicz-pieńkowska
- Department of Physical Chemistry, Medical University of Gdańsk, Al. Gen. Hallera 107, 80-416, Gdańsk, Poland
-
Jarosław Sączewski
- Department of Organic Chemistry, Faculty of Pharmacy, Medical University of Gdańsk, Al. Gen. J. Hallera 107, 80-416, Gdańsk, Poland
Cite as
Full text
- Publication version
- Accepted or Published Version
- DOI:
- Digital Object Identifier (open in new tab) 10.1038/s41598-022-19262-w
- License
-
open in new tab
Keywords
Details
- Category:
- Articles
- Type:
- artykuły w czasopismach
- Published in:
-
Scientific Reports
no. 12,
ISSN: 2045-2322 - Language:
- English
- Publication year:
- 2022
- Bibliographic description:
- Fedorowicz J., Bazar D., Brankiewicz W., Kapica H., Ciura K., Zalewska-Piątek B., Piątek R., Cal K., Mojsiewicz-Pieńkowska K., Sączewski J.: Development of Safirinium dyes for new applications: fluorescent staining of bacteria, human kidney cells, and the horny layer of the epidermis// Scientific Reports -Vol. 12,iss. 1 (2022), s.15098-
- DOI:
- Digital Object Identifier (open in new tab) 10.1038/s41598-022-19262-w
- Sources of funding:
-
- Free publication
- Verified by:
- Gdańsk University of Technology
seen 109 times
Recommended for you
Application of safirinium N-hydroxysuccinimide esters to derivatization of peptides for high-resolution mass spectrometry, tandem mass spectrometry, and fluorescent labeling of bacterial cells
- J. Fedorowicz,
- M. Wierzbicka,
- M. Cebrat
- + 5 authors
Multispectral Imaging Using Fluorescent Properties of Indocyanine Green and Methylene Blue in Colorectal Surgery—Initial Experience
- W. Polom,
- M. Migaczewski,
- J. Skokowski
- + 6 authors
Applications of nanosorbents in dispersive solid phase extraction/microextraction approaches for monitoring of synthetic dyes in various types of samples: A review
- W. A. Khan,
- P. Varanusupakul,
- H. Haq
- + 2 authors