In Vitro Studies on Nanoporous, Nanotubular and Nanosponge-Like Titania Coatings, with the Use of Adipose-Derived Stem Cells - Publication - MOST Wiedzy


In Vitro Studies on Nanoporous, Nanotubular and Nanosponge-Like Titania Coatings, with the Use of Adipose-Derived Stem Cells


In vitro biological research on a group of amorphous titania coatings of different nanoarchitectures (nanoporous, nanotubular, and nanosponge-like) produced on the surface of Ti6Al4V alloy samples have been carried out, aimed at assessing their ability to interact with adipose-derived mesenchymal stem cells (ADSCs) and affect their activity. The attention has been drawn to the influence of surface coating architecture and its physicochemical properties on the ADSCs proliferation. Moreover, in vitro co-cultures: (1) fibroblasts cell line L929/ADSCs and (2) osteoblasts cell line MG-63/ADSCs on nanoporous, nanotubular and nanosponge-like TiO2 coatings have been studied. This allowed for evaluating the impact of the surface properties, especially roughness and wettability, on the creation of the beneficial microenvironment for co-cultures and/or enhancing differentiation potential of stem cells. Obtained results showed that the nanoporous surface is favorable for ADSCs, has great biointegrative properties, and supports the growth of co-cultures with MG-63 osteoblasts and L929 fibroblasts. Additionally, the number of osteoblasts seeded and cultured with ADSCs on TNT5 surface raised after 72-h culture almost twice when compared with the unmodified scaffold and by 30% when compared with MG-63 cells growing alone. The alkaline phosphatase activity of MG-63 osteoblasts co-cultured with ADSCs increased, that indirectly confirmed our assumptions that TNT-modified scaffolds create the osteogenic niche and enhance osteogenic potential of ADSCs.


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artykuły w czasopismach
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Materials no. 13, pages 1 - 18,
ISSN: 1996-1944
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Bibliographic description:
Ehlert M., Radtke A., Jędrzejewski T., Roszek K., Bartmański M., Piszczek P.: In Vitro Studies on Nanoporous, Nanotubular and Nanosponge-Like Titania Coatings, with the Use of Adipose-Derived Stem Cells// Materials -Vol. 13,iss. 5 (2020), s.1-18
Digital Object Identifier (open in new tab) 10.3390/ma13071574
Bibliography: test
  1. Roach, P.; Eglin, D.; Rohde, K.; Perry, C.C. Modern biomaterials: A review-Bulk properties and implications of surface modifications. J. Mater. Sci. Mater. Med. 2007, 18, 1263-1277. [CrossRef] [PubMed] open in new tab
  2. Kaur, M.; Singh, K. Review on titanium and titanium based alloys as biomaterials for orthopaedic applications. Mater. Sci. Eng. C 2019, 102, 844-862. [CrossRef] [PubMed] open in new tab
  3. Navarro, M.; Michiardi, A.; Castano, O.; Planell, J. Biomaterials in orthopaedics. J. R. Soc. Interface 2008, 5, 1137-1158. [CrossRef] [PubMed] open in new tab
  4. Rehman, M.; Madni, A.; Webster, T.J. The era of biofunctional biomaterials in orthopedics: What does the future hold? Expert Rev. Med. Devices 2018, 15, 193-204. [CrossRef] [PubMed] open in new tab
  5. Geetha, M.; Singh, A.K.; Asokamani, R.; Gogia, A.K. Ti based biomaterials, the ultimate choice for orthopaedic implants-A review. Prog. Mater. Sci. 2009, 54, 397-425. [CrossRef] open in new tab
  6. Liu, X.; Chu, P.K.; Ding, C. Surface modification of titanium, titanium alloys, and related materials for biomedical applications. Mater. Sci. Eng. R Rep. 2004, 47, 49-121. [CrossRef] open in new tab
  7. Swami, N.; Cui, Z.; Nair, L.S. Titania nanotubes: Novel nanostructures for improved osseointegration. J. Heat Transf. 2011, 133. [CrossRef] open in new tab
  8. Minagar, S.; Berndt, C.C.; Wang, J.; Ivanova, E.; Wen, C. A review of the application of anodization for the fabrication of nanotubes on metal implant surfaces. Acta Biomater. 2012, 8, 2875-2888. [CrossRef] open in new tab
  9. Kodama, A.; Bauer, S.; Komatsu, A.; Asoh, H.; Ono, S.; Schmuki, P. Bioactivation of titanium surfaces using coatings of TiO 2 nanotubes rapidly pre-loaded with synthetic hydroxyapatite. Acta Biomater. 2009, 5, 2322-2330. [CrossRef] open in new tab
  10. Veronovski, N. TiO 2 Applications as a Function of Controlled Surface Treatment. In Titanium Dioxide-Material for a Sustainable Environment; open in new tab
  11. Yang, D., Ed.; IntechOpen Ltd.: London, UK, 2018; Volume 21, pp. 421-443. open in new tab
  12. Mydin, R.B.S.M.N.; Hazan, R.; FaridWajidi, M.F.; Sreekantan, S. Titanium Dioxide Nanotube Arrays for Biomedical Implant Materials and Nanomedicine Applications. In Titanium Dioxide-Material for a Sustainable Environment; open in new tab
  13. Yang, D., Ed.; IntechOpen Ltd.: London, UK, 2018; Volume 23, pp. 469-483. open in new tab
  14. Sulka, G.D.; Kapusta-Kołodziej, J.; Brzózka, A.; Jaskuła, M. Fabrication of nanoporous TiO 2 by electrochemical anodization. Electrochim. Acta 2010, 55, 4359-4367. [CrossRef] open in new tab
  15. Saharudin, K.A.; Sreekantan, S.; Aziz, S.N.Q.A.A.; Hazan, R.; Lai, C.W.; Mydin, R.B.S.M.N.; Mat, I. Surface modification and bioactivity of anodic Ti6Al4V alloy. J. Nanosci. Nanotechnol. 2013, 13, 1696-1705. [CrossRef] [PubMed] open in new tab
  16. Saharudin, K.A.; Sreekantan, S.; Mydin, R.B.S.N.M.; Basiron, N.; Krengvirat, W. Factor Affecting Geometry of TiO 2 Nanotube Arrays (TNAs) in Aqueous and Organic Electrolyte. In Titanium Dioxide-Material for a Sustainable Environment; open in new tab
  17. Yang, D., Ed.; IntechOpen Ltd.: London, UK, 2018; Volume 6, pp. 117-130.
  18. Macak, J.M.; Tsuchiya, H.; Ghicov, A.; Yasuda, K.; Hahn, R.; Bauer, S.; Schmuki, P. TiO 2 nanotubes: Self-organized electrochemical formation, properties and applications. Curr. Opin. Solid State Mater. Sci. 2007, 11, 3-18. [CrossRef] open in new tab
  19. Mor, G.K.; Varghese, O.K.; Paulose, M.; Shankar, K.; Grimes, C.A. A review on highly ordered, vertically oriented TiO 2 nanotube arrays: Fabrication, material properties, and solar energy applications. Sol. Energy Mater. Sol. Cells 2006, 90, 2011-2075. [CrossRef] open in new tab
  20. Wang, Q.; Huang, J.Y.; Li, H.Q.; Chen, Z.; Zhao, A.Z.J.; Wang, Y.; Zhang, K.Q.; Sun, H.T.; Al-Deyab, S.S.; Lai, Y.K. TiO 2 nanotube platforms for smart drug delivery: A review. Int. J. Nanomed. 2016, 11, 4819-4834.
  21. Jonášová, L.; Müller, F.A.; Helebrant, A.; Strnad, J.; Greil, P. Biomimetic apatite formation on chemically treated titanium. Biomaterials 2005, 25, 1187-1194. [CrossRef] open in new tab
  22. Hayakawa, S.; Okamoto, K.; Yoshioka, T. Accelerated induction of in vitro apatite formation by parallel alignment of hydrothermally oxidized titanium substrates separated by sub-millimeter gaps. J. Asian Ceram. Soc. 2019, 7, 90-100. [CrossRef] open in new tab
  23. Radtke, A.; Piszczek, P.; Topolski, A.; Lewandowska,Ż.; Talik, E.; Hald Andersen, I.; Nielsen, L.P.; Heikkilä, M.; Leskelä, M. The structure and the photocatalytic activity of titania nanotube and nanofiber coatings. Appl. Surf. Sci. 2016, 368, 165-172. [CrossRef] open in new tab
  24. Radtke, A.; Topolski, A.; Jędrzejewski, T.; Kozak, W.; Sadowska, B.; Wieckowska-Szakiel, M.; Piszczek, P. Bioactivity Studies on Titania Coatings and the Estimation of Their Usefulness in the Modification of Implant Surfaces. Nanomaterials 2017, 7, 90. [CrossRef] open in new tab
  25. Radtke, A.; Topolski, A.; Jędrzejewski, T.; Sadowska, B.; Więckowska-Szakiel, M.; Szubka, M.; Talik, E.; Nielsen, L.P.; Piszczek, P. Studies on the bioactivity and photocatalytic properties of titania nanotube coatings produced with the use of the low potential anodization of Ti6Al4V alloy surface. Nanomaterials 2017, 7, 197. [CrossRef] open in new tab
  26. Radtke, A.; Bal, M.; Jędrzejewski, T. Novel titania nanocoatings produced by the anodic anodization with the use of the cyclically changing potential; their photocatalytic activity and biocompability. Nanomaterials 2018, 8, 712. [CrossRef] open in new tab
  27. Radtke, A.; Ehlert, M.; Bartmański, M.; Jędrzejewski, T. The morphology, structure, mechanical properties and biocompatibility of nanotubular titania coatings before and after autoclaving process. J. Clin. Med. 2019, 8, 272. [CrossRef] open in new tab
  28. Radtke, A. Photocatalytic activity of nanostructured titania films obtained by electrochemical, chemical, and thermal oxidation of Ti6Al4V alloy-Comparative analysis. Catalysts 2019, 9, 279. [CrossRef] open in new tab
  29. Radtke, A.; Ehlert, M.; Jędrzejewski, T.; Sadowska, B.; Więckowska-Szakiel, M.; Holopainen, J.; Ritala, M.; Leskela, M.; Bartmański, M.; Szkodo, M.; et al. Titania Nanotubes/Hydroxyapatite Nanocomposites Produced with the Use of the Atomic Layer Deposition Technique: Estimation of Bioactivity and Nanomechanical Properties. Nanomaterials 2019, 9, 123. [CrossRef] [PubMed] open in new tab
  30. Radtke, A.; Jędrzejewski, T.; Kozak, W.; Sadowska, B.; Więckowska-Szakiel, M.; Talik, E.; Mäkelä, M.; Leskelä, M.; Piszczek, P. Optimization of the silver clusters PEALD process on the surface of 1-D titania coatings. Nanomaterials 2017, 7, 193. [CrossRef] [PubMed] open in new tab
  31. Piszczek, P.; Lewandowska,Ż.; Radtke, A.; Jędrzejewski, T.; Kozak, W.; Sadowska, B.; Szubka, M.; Talik, E.; Fiori, F. Biocompatibility of Titania Nanotube Coatings Enriched with Silver Nanograins by Chemical Vapor Deposition. Nanomaterials 2017, 7, 274. [CrossRef] [PubMed] open in new tab
  32. Radtke, A.; Grodzicka, M.; Ehlert, M.; Muzioł, T.; Szkodo, M.; Bartmański, M.; Piszczek, P. Studies on silver ions releasing processes and mechanical properties of surface-modified titanium alloy implants. Int. J. Mol. Sci. 2018, 19, 3962. [CrossRef] open in new tab
  33. Radtke, A.; Grodzicka, M.; Ehlert, M.; Jędrzejewski, T.; Wypij, M.; Golińska, P. "To be microbiocidal and not to be cytotoxic at the same time . . . "-Silver nanoparticles in their main role on the surface of titanium alloy implants. J. Clin. Med. 2019, 8, 334. [CrossRef] open in new tab
  34. Marini, F.; Luzi, E.; Fabbri, S.; Ciuffi, S.; Sorace, S.; Tognarini, I.; Galli, G.; Zonefrati, R.; Sbaiz, F.; Brandi, M.L. Osteogenic differentiation of adipose tissue-derived mesenchymal stem cells on nanostructured Ti6Al4V and Ti13Nb13Zr. Clin. Cases Miner. Bone Metab. 2015, 12, 224-237. [CrossRef] open in new tab
  35. Dias-Netipanyj, M.F.; Cowden, K.; Sopchenski, L.; Cogo, S.C.; Elifio-Esposito, S.; Popat, K.C.; Soares, P. Effect of crystalline phases of titania nanotube arrays on adipose derived stem cell adhesion and proliferation. Mater. Sci. Eng. C 2019, 103, 109850. [CrossRef] open in new tab
  36. Bressan, E.; Sbricoli, L.; Guazzo, R.; Tocco, I.; Roman, M.; Vindigni, V.; Stellini, E.; Gardin, C.; Ferroni, L.; Sivolella, S.; et al. Nanostructured Surfaces of Dental Implants. Int. J. Mol. Sci. 2013, 14, 1918-1931. [CrossRef] open in new tab
  37. Martino, S.; D'Angelo, F.; Armentano, I.; Kenny, J.M.; Orlacchio, A. Stem cell-biomaterial interactions for regenerative medicine. Biotechnol. Adv. 2012, 30, 338-351. [CrossRef] [PubMed] open in new tab
  38. Cowden, K.; Dias-Netipanyj, M.F.; Popat, K.C. Adhesion and Proliferation of Human Adipose-Derived Stem Cells on Titania Nanotube Surfaces. Regen. Eng. Transl. Med. 2019, 5, 435-445. [CrossRef] open in new tab
  39. Ciuffi, S.; Zonefrati, R.; Brandi, M.L. Adipose stem cells for bone tissue repair. Clin. Cases Miner. Bone Metab. 2017, 14, 217-226. [CrossRef] [PubMed] open in new tab
  40. Lavenus, S.; Berreur, M.; Trichet, V.; Pilet, P.; Louarn, G.; Layrolle, P. Adhesion and osteogenic differentiation of human mesenchymal stem cells on titanium nanopores. Eur. Cells Mater. 2011, 22, 84-96. [CrossRef] [PubMed] open in new tab
  41. Caplan, A.I.; Dennis, J.E. Mesenchymal stem cells as trophic mediators. J. Cell. Biochem. 2006, 98, 1076-1084. [CrossRef] [PubMed] open in new tab
  42. Dominici, M.; Le Blanc, K.; Mueller, I.; Slaper-Cortenbach, I.; Marini, F.; Krause, D.; Deans, R.; Keating, A.; Prockop, D.; Horwitz, E. Minimal criteria for defining multipotent mesenchymal stromal cells. Cytotherapy 2006, 8, 315-317. [CrossRef] [PubMed] open in new tab
  43. Vercellino, M.; Ceccarelli, G.; Cristofaro, F.; Balli, M.; Bertoglio, F.; Bruni, G.; Benedetti, L.; Avanzini, M.A.; Imbriani, M.; Visai, L. Nanostructured TiO 2 Surfaces Promote Human Bone Marrow Mesenchymal Stem Cells Differentiation to Osteoblasts. Nanomaterials 2016, 6, 124. [CrossRef] open in new tab
  44. James, A.W.; Zara, J.N.; Zhang, X.; Askarinam, A.; Goyal, R.; Chiang, M.; Yuan, W.; Chang, L.; Corselli, M.; Shen, J.; et al. Perivascular stem cells: A prospectively purified mesenchymal stem cell population for bone tissue engineering. Stem Cells Transl. Med. 2012, 1, 510-519. [CrossRef] open in new tab
  45. Caplan, A.I. Mesenchymal stem cells. J. Orthop. Res. 1991, 9, 641-650. [CrossRef] open in new tab
  46. Lindroos, B.; Suuronen, R.; Miettinen, S. The potential of adipose stem cells in regenerative medicine. Stem Cell Rev. Rep. 2011, 7, 269-291. [CrossRef] open in new tab
  47. Mazini, L.; Rochette, L.; Amine, M.; Malka, G. Regenerative Capacity of Adipose Derived Stem Cells (ADSCs), Comparison with Mesenchymal Stem Cells (MSCs). Int. J. Mol. Sci. 2019, 20, 2523. [CrossRef] [PubMed] open in new tab
  48. Gimble, J.M.; Katz, A.J.; Bunnell, B.A. Adiposed-derived stem cells for regenerative medicine. Circ. Res. 2007, 100, 1249-1260. [CrossRef] [PubMed] open in new tab
  49. Hattori, H.; Sato, M.; Masuoka, K.; Ishihara, M.; Kikuchi, T.; Matsui, T.; Takase, B.; Ishizuka, T.; Kikuchi, M.; Fujikawa, K.; et al. Osteogenic potential of human adipose tissue derived stromal cells as an alternative stem cell source. Cells Tissues Organs 2004, 178, 2-12. [CrossRef] [PubMed] open in new tab
  50. De Ugarte, D.A.; Morizono, K.; Elbarbary, A.; Alfonso, Z.; Zuk, P.A.; Zhu, M.; Dragoo, J.L.; Ashjian, P.; Thomas, B.; Benhaim, P.; et al. Comparison of multi-lineage cell from human adipose tissue and bone marrow. Cells Tissues Organs 2003, 174, 101-109. [CrossRef] open in new tab
  51. Bunnell, B.A.; Flaat, M.; Gagliardi, C.; Patel, B.; Ripoll, C. Adipose-derived stem cells: Isolation, expansion and differentiation. Methods 2008, 45, 115-120. [CrossRef] open in new tab
  52. Lewallen, E.A.; Jones, D.L.; Dudakovic, A.; Thaler, R.; Paradise, C.R.; Kremers, H.M.; Abdel, M.P.; Kakar, S.; Dietz, A.B.; Cohene, R.C.; et al. Osteogenic potential of human adipose-tissue-derived mesenchymal stromal cells cultured on 3D-printed porous structured titanium. Gene 2016, 581, 95-106. [CrossRef] open in new tab
  53. Cowden, K.; Dias-Netipanyj, M.F.; Popat, K.C. Effects of titania nanotube surfaces on osteogenic differentiation of human adipose-derived stem cells. Nanomed. Nanotechnol. Biol. Med. 2019, 17, 380-390. [CrossRef] open in new tab
  54. Ehlert, M.; Roszek, K.; Jędrzejewski, T.; Bartmański, M.; Radtke, A. Titania Nanofiber Scaffolds with Enhanced Biointegration Activity-Preliminary in vitro Studies. Int. J. Mol. Sci. 2019, 20, 5642. [CrossRef] open in new tab
  55. Yuan, Y.; Lee, T.R. Chapter 1 Contact Angle and Wetting Properties. In Surface Science Techniques, Springer Series in Surface Sciences; Bracco, G., Holst, B., Eds.; Springer: Berlin/Heidelberg, Germany, 2013; pp. 3-34. open in new tab
  56. Oliver, W.C.; Pharr, G.M. An improved technique for determining hardness and elastic modulus using load and displacement sensing indentation experiments. J. Mater. Res. 1992, 7, 1564-1583. [CrossRef] open in new tab
  57. Zhang, W.F.; He, Y.L.; Zhang, M.S.; Yin, Z.; Chen, Q. Raman scattering study on anatase TiO 2 nanocrystals. J. Phys. D Appl. Phys. 2000, 33, 912. [CrossRef] open in new tab
  58. Mazza, T.; Barborini, E.; Piseri, P.; Milani, P.; Cattaneo, D.; Li Bassi, A.; Bottani, C.E.; Ducati, C. Raman spectroscopy characterization of TiO 2 rutile nanocrystals. Phys. Rev. B 2007, 75, 045416. [CrossRef] open in new tab
  59. Chen, X.; Mao, S.S. Titanium dioxide nanomaterials: Synthesis, properties, modifications and applications. Chem. Rev. 2007, 107, 2891-2959. [CrossRef] [PubMed] open in new tab
  60. Hardcastle, F. Raman Spectroscopy of Titania (TiO 2 ) Nanotubular Water-Splitting Catalysts. J. Ark. Acad. Sci. 2011, 65, 43-48. open in new tab
  61. Busani, T.; Devine, R.A.B. Dielectric and infrared properties of TiO 2 films containing anatase and rutile. Semicond. Sci. Technol. 2005, 20, 870. [CrossRef] open in new tab
  62. Coy, E.; Yate, L.; Kabacińska, Z.; Jancelewicz, M.; Jurga, S.; Iatsunskyi, I. Topographic reconstruction and mechanical analysis of atomic layer deposited Al 2 O 3 /TiO 2 nanolaminates by nanoindentation. Mater. Des. 2016, 111, 584-591. [CrossRef] open in new tab
  63. Bartmanski, M.; Zielinski, A.; Jazdzewska, M.; Głodowska, J.; Kalka, P. Effects of electrophoretic deposition times and nanotubular oxide surfaces on properties of the nanohydroxyapatite/nanocopper coating on the Ti1 3 Zr1 3 Nb alloy. Ceram. Int. 2019, 45, 20002-20010. [CrossRef] open in new tab
  64. Roach, P.; Eglin, D.; Ronde, K.; Perry, C.C.J. Surface Tailoring for Controlled Protein Adsorption: Effect of Topography at the Nanometer Scale and Chemistry. Mater. Sci. Mater. Med. 2007, 18, 1263-1277. [CrossRef] open in new tab
  65. Comelles, J.; Estévez, M.; Martínez, E.; Samitier, J. The role of surface energy of technical polymers in serum protein adsorption and MG-63 cells adhesion. Nanomed. Nanotechnol. Biol. Med. 2010, 6, 44-51. [CrossRef] open in new tab
  66. Tanaka, M. Design of novel 2D and 3D biointerfaces using self-organization to control cell behaviour. Biochim. Biophys. Acta 2011, 1810, 251-258. [CrossRef] open in new tab
  67. Yang, Y.; Leong, K.W. Nanoscale surfacing for regenerative medicine. WIRES Nanomed. Nanobiotechnol. 2010, 2, 478-495. [CrossRef] open in new tab
  68. Ricci, J.L.; Grew, J.C.; Alexander, H. Connective-tissue responses to defined biomaterial surfaces. I. Growth of rat fibroblast and bone marrow cell colonies on microgrooved substrates. J. Biomed. Mater. Res. A 2007, 85, 313-325. [CrossRef] [PubMed] open in new tab
  69. Kunzler, T.P.; Drobek, T.; Schuler, M. Spencer, Systematic study of osteoblast and fibroblast response to roughness by means of surface-morphology gradients. Biomaterials 2007, 28, 2175-2182. [CrossRef] [PubMed] open in new tab
  70. Łopacińska, J.M.; Gradinaru, C.; Wierzbicki, R.; Købler, C.; Schmidt, M.S.; Madsen, M.T.; Skolimowski, M.; Dufva, M.; Flyvbjerg, H.; Mølhave, K. Cell motility, morphology, viability and proliferation in response to nanotopography on silicon black. Nanoscale 2012, 4, 3739-3745. [CrossRef] [PubMed] open in new tab
  71. Takamori, E.R.; Cruz, R.; Gonçalvez, F.; Zanetti, R.V.; Zanetti, A.; Granjeiro, J.M. Effect of roughness of zirconia and titanium on fibroblast adhesion. Artif. Organs 2008, 32, 305-309. [CrossRef] [PubMed] open in new tab
  72. Huang, Y.; Hao, M.; Nian, X.; Qiao, H.; Zhang, X.; Zhang, X.; Song, G.; Guo, J.; Pang, X.; Zhang, H. Strontium and copper co-substituted hydroxyapatite-based coatings with improved antibacterial activity and cytocompatibility fabricated by electrodeposition. Ceram. Int. 2016, 42, 11876-11888. [CrossRef] open in new tab
  73. Mohan, L.; Durgalakshmi, D.; Geetha, M.; Sankara Narayanan, T.S.N.; Asokamani, R. Electrophoretic deposition of nanocomposite (HAp+TiO 2 ) on titanium alloy for biomedical applications. Ceram. Int. 2012, 38, 3435-3443. [CrossRef] open in new tab
  74. Rautray, T.R.; Narayanan, R.; Kim, K.H. Ion implantation of titanium based biomaterials. Prog. Mater. Sci. 2011, 56, 1137-1177. [CrossRef] open in new tab
  75. Gross, K.A.; Babovic, M. Influence of abrasion on the surface characteristics of thermally sprayed hydroxyapatitae coatings. Biomaterials 2002, 23, 4731-4737. [CrossRef] open in new tab
  76. Alam, F.; Balani, K. Adhesion force of staphylococcus aureus on various biomaterial surfaces. J. Mech. Behav. Biomed. Mater. 2017, 65, 872-880. [CrossRef] open in new tab
  77. Ribeiro, M.; Monteiro, F.J.; Ferraz, M.P. Infection of orthopedic implants with emphasis on bacterial adhesion process and techniques used in studying bacterial-material interactions. BioMatter 2012, 2, 176-194. [CrossRef] open in new tab
  78. Kubacka, A.; Diez, M.S.; Rojo, D.; Bargiela, R.; Ciordia, S.; Zapico, I.; Albar, J.P.; Barbas, C.; Martins Dos Santos, V.A.P.; Fernández-García, M.; et al. Understanding the antimicrobial mechanism of TiO 2 -based nanocomposite films in a pathogenic bacterium. Sci. Rep. 2014, 4, 4134. [CrossRef] [PubMed] open in new tab
  79. Herrmann, H.; Bär, H.; Kreplak, L.; Strelkov, S.V.; Aebi, U. Intermediate filaments: From cell architecture to nanomechanics. Nat. Rev. Mol. Cell Biol. 2007, 8, 562-573. [CrossRef] [PubMed] open in new tab
  80. Corni, I.; Ryan, M.P.; Boccaccini, A.R. Electrophoretic deposition: From traditional ceramics to nanotechnology. J. Eur. Ceram. Soc. 2008, 28, 1353-1367. [CrossRef] open in new tab
  81. Von Wilmowsky, C.; Bauer, S.; Lutz, R.; Meisel, M.; Neukam, F.W.; Toyoshima, T.; Schmuki, P.; Nkenke, E.; Schlegel, K.A. In Vivo Evaluation of Anodic TiO 2 Nanotubes; An Experimental Study in the Pig. J. Biomed. Mater. Res. Part B Appl. Biomater. 2009, 89, 165-171. [CrossRef] open in new tab
  82. Kim, D.; Lee, K.; Roy, P.; Birajdar, B.I.; Spiecker, E.; Schmuki, P. Formation of a non-thickness-limited titanium dioxide mesosponge and its use in dye-sensitized solar cells. Angew. Chem. Int. Ed. 2009, 48, 9326-9329. [CrossRef] open in new tab
  83. Noyama, Y.; Miura, T.; Ishimoto, T.; Itaya, T.; Niinomi, M.; Nakano, T. Bone loss and reduced bone quality of the human femur after total hip arthroplasty under stress-shielding effects by titanium-based implant. Mater. Trans. 2012, 53, 565-570. [CrossRef] open in new tab
  84. Asgharzadeh Shirazi, H.; Ayatollahi, M.R.; Asnafi, A. To reduce the maximum stress and the stress shielding effect around a dental implant-bone interface using radial functionally graded biomaterials. Comput. Methods Biomech. Biomed. Eng. 2017, 20, 750-759. [CrossRef] open in new tab
  85. Huiskes, R.; Weinans, H.; Van Rietbergen, B. The relationship between stress shielding and bone resorption around total hip stems and the effects of flexible materials. Clin. Orthop. Relat. Res. 1992, 274, 124-134. [CrossRef] open in new tab
  86. Piszczek, P.; Radtke, A.; Ehlert, M.; Jędrzejewski, T.; Sznarkowska, A.; Sadowska, B.; Bartmański, M.; Erdogan, Y.K.; Ercan, B.; Jędrzejczyk, W. Comprehensive Evaluation of the Biological Properties of Surface-Modified Titanium Alloy Implants. J. Clin. Med. 2020, 9, 342. [CrossRef] open in new tab
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