Simultaneous optical and electrochemical label-free biosensing with ITO-coated lossy-mode resonance sensor - Publication - Bridge of Knowledge

Search

Simultaneous optical and electrochemical label-free biosensing with ITO-coated lossy-mode resonance sensor

Abstract

In this work we discuss a new label-free biosensing device based on indium tin oxide (ITO) overlaid section of a multimode optical fiber fused silica core. The sensor has been used to optical measurements also simultaneously interrogated electrochemically (EC). Due to optimized thickness and optical properties of ITO film, a lossy-mode resonance (LMR) could be observed in the optical domain, where electrical properties of the film allowed for application of the sensor as a working electrode in an EC setup. It has been confirmed that the LMR response depends on optical properties of the external medium, as well as potential applied to the electrode during cyclic voltammetry. After the ITO surface functionalization with amine groups and covalently attached biotin, the device has been applied for label-free biosensing of avidin in both the domains simultaneously. On the example of biotin-avidin detection system it was demonstrated that when avidin concentration increases a decrease in current and increase in LMR wavelength shift were recorded in EC and optical domain, respectively. Both optical and EC responses follow the protein interaction process, and thus can be used as cross-verification of the readouts. Moreover, an extended information has been achieved comparing to solely EC interrogation, i.e., the grafting process of biotin and avidin was directly monitored optically displaying individual steps of an incubation procedure.

Citations

  • 4 0

    CrossRef

  • 0

    Web of Science

  • 4 0

    Scopus

Authors (8)

Cite as

Full text

download paper
downloaded 72 times
Publication version
Accepted or Published Version
License
Creative Commons: CC-BY-NC-ND open in new tab

Keywords

Details

Category:
Articles
Type:
artykuły w czasopismach
Published in:
BIOSENSORS & BIOELECTRONICS no. 154,
ISSN: 0956-5663
Language:
English
Publication year:
2020
Bibliographic description:
Śmietana M., Koba M., Sezemsky P., Szot-Karpińska K., Burnat D., Stranak V., Niedziółka-Jönsson J., Bogdanowicz R.: Simultaneous optical and electrochemical label-free biosensing with ITO-coated lossy-mode resonance sensor// BIOSENSORS & BIOELECTRONICS -Vol. 154, (2020), s.112050-
DOI:
Digital Object Identifier (open in new tab) 10.1016/j.bios.2020.112050
Verified by:
Gdańsk University of Technology

seen 86 times

Recommended for you

Meta Tags