Search results for: ASPARTIC PROTEASE

• The psychrotrophic yeast Sporobolomyces roseus LOCK 1119 as a source of a highly active aspartic protease for the in vitro production of antioxidant peptides

  Publication

  • A. Białkowska
  • J. Krysiak
  • T. Florczak
  • K. Szulczewska
  • M. Wanarska
  • M. Turkiewicz

  - BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY - Year 2018

  A psychrotrophic yeast strain producing a cold-adapted protease at low temperature was classified as Sporobolomyces roseus. In standard YPG medium, S. roseus LOCK 1119 synthesized an extracellular protease with an activity of approximately 560 U/L. Optimization of medium composition and process temperature considerably enhanced enzyme biosynthesis; an approximate 70% increase in activity (2060 U/L). The native enzyme was purified...

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• Organic solvents aggregating and shaping structural folding of protein, a case study of the protease enzyme

  Publication

  • M. Padariya
  • M. Bagiński
  • M. Babak
  • U. Kalathiya

  - BIOPHYSICAL CHEMISTRY - Year 2022

  Low solubility of reactants or products in aqueous solutions can result in the enzymatic catalytic reactions that can occur in non-aqueous solutions. In current study we investigated aqueous solutions containing different organic solvents / deep eutectic solvents (DESs) that can influence the protease enzyme's activity, structural, and thermal stabilities. Retroviral aspartic protease enzyme is responsible for the cleavage of the...

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