The psychrotrophic yeast Sporobolomyces roseus LOCK 1119 as a source of a highly active aspartic protease for the in vitro production of antioxidant peptides

Aneta Białkowska; Joanna Krysiak; Tomasz Florczak; Katarzyna Szulczewska; Marta Wanarska; Marianna Turkiewicz

doi:10.1002/bab.1656

The psychrotrophic yeast Sporobolomyces roseus LOCK 1119 as a source of a highly active aspartic protease for the in vitro production of antioxidant peptides

Abstract

A psychrotrophic yeast strain producing a cold-adapted protease at low temperature was classiﬁed as Sporobolomyces roseus. In standard YPG medium, S. roseus LOCK 1119 synthesized an extracellular protease with an activity of approximately 560 U/L. Optimization of medium composition and process temperature considerably enhanced enzyme biosynthesis; an approximate 70% increase in activity (2060 U/L). The native enzyme was puriﬁed to homogeneity by cation exchange chromatography followed by a size exclusion step, resulting in a 103-fold increase in speciﬁc activity (660 U/mg) with 25% recovery. The enzyme displayed 10%–30% of its maximum activity at 0–25 C, with the optimum temperature being 50 C. Protease G8 was strongly inactivated by pepstatin A, an aspartic protease inhibitor. The enzyme was used to hydrolyze four natural substrates, and their antioxidant activities were evaluated against 1,1-diphenyl-2-picrylhydrazyl. The highest antioxidant activity (69%) was recorded for beef casein.

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Authors (6)

Aneta Białkowska
- Politechnika Łódzka Instytut Biochemii Technicznej
Joanna Krysiak
- Politechnika Łódzka Instytut Biochemii Technicznej
Tomasz Florczak dr inż.
- Politechnika Łódzka Instytut Biochemii technicznej
Katarzyna Szulczewska
- Politechnika Łódzka Instytut Biochemii Technicznej
Marta Wanarska dr hab. inż.
Marianna Turkiewicz prof. dr hab.
- Politechnika Łódzka Instytut Biochemii Technicznej

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Articles

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artykuł w czasopiśmie wyróżnionym w JCR

Published in:

BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY no. 65, pages 726 - 738,
ISSN: 0885-4513

Language:

English

Publication year:

2018

Bibliographic description:

Białkowska A., Krysiak J., Florczak T., Szulczewska K., Wanarska M., Turkiewicz M.: The psychrotrophic yeast Sporobolomyces roseus LOCK 1119 as a source of a highly active aspartic protease for the in vitro production of antioxidant peptides// BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY. -Vol. 65, nr. 5 (2018), s.726-738

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