Search results for: SAFIRINIUM DYES

• Development of Safirinium dyes for new applications: fluorescent staining of bacteria, human kidney cells, and the horny layer of the epidermis

  Publication

  • J. Fedorowicz
  • D. Bazar
  • W. Brankiewicz
  • H. Kapica
  • K. Ciura
  • B. Zalewska-Piątek
  • R. Piątek
  • K. Cal
  • K. Mojsiewicz-pieńkowska
  • J. Sączewski

  - Scientific Reports - Year 2022

  Low-molecular synthetic fluorophores are convenient tools in bioimaging applications. Several derivatives of Safirinium dyes as well as their reactive N-hydroxysuccinimide (NHS) esters bearing diverse substituents were synthesized and evaluated experimentally in terms of their lipophilicity by means of reverse-phase and immobilized artificial membrane high-performance liquid chromatography. Subsequently, the selected compounds...

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• Application of safirinium N-hydroxysuccinimide esters to derivatization of peptides for high-resolution mass spectrometry, tandem mass spectrometry, and fluorescent labeling of bacterial cells

  Publication

  • J. Fedorowicz
  • M. Wierzbicka
  • M. Cebrat
  • P. Wiśniewska
  • R. Piątek
  • B. Zalewska-Piątek
  • Z. Szewczuk
  • J. Sączewski

  - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES - Year 2020

  Mass spectrometry methods are commonly used in the identification of peptides and biomarkers. Due to a relatively low abundance of proteins in biological samples, there is a need for the development of novel derivatization methods that would improve MS detection limits. Hence, novel fluorescent N-hydroxysuccinimide esters of dihydro-[1,2,4]triazolo[4,3-a]pyridin-2-ium carboxylates (Safirinium P dyes) have been synthesized. The...

  Full text available to download