Search results for: ultracentrifugation

• Quality Control of Bacterial Extracellular Vesicles with Total Protein Content Assay, Nanoparticles Tracking Analysis, and Capillary Electrophoresis

  Publication

  • A. Steć
  • J. Jońca
  • K. Waleron
  • M. Waleron
  • A. Płoska
  • L. Kalinowski
  • B. Wielgomas
  • S. Dziomba

  - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES - Year 2022

  Extracellular vesicles (EVs) were isolated from Pectobacterium zantedeschiae culturing media using direct ultracentrifugation (UC), iodixanol cushion ultracentrifugation (ICUC), and iodixanol density gradient ultracentrifugation (IDGUC) techniques. The isolates were characterized with total protein content assay (bicinchoninic acid assay, BCA), nanoparticles tracking analysis (NTA), and capillary electrophoresis (CE). A satisfactory...

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• Direct injection liquid chromatography-mass spectrometry (DI-LC-MS) analysis for rapid lipidomic profiling of extracellular vesicles

  Publication

  • W. Hewelt-Belka
  • M. Młynarczyk
  • J. Matinha-Cardoso
  • F. Gajdowska
  • P. Oliveira
  • D. Gutowska-Owsiak

  - Year 2023

  Extracellular vesicles (EVs) are small, spherical particles produced by eukaryotic and prokaryotic cells, surrounded by a bilayer membrane and carrying various bioactive molecules, such as proteins, surface receptors, membrane and soluble proteins, lipids, and nucleic acids. EVs are of substantial interest because of their important roles in cell communication, epigenetic regulation and possible application in disease diagnosis...

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