Barbara Michalska - Publications - Bridge of Knowledge

Barbara Michalska

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Year 2016

5-Bromo-2′-deoxycytidine—a potential DNA photosensitizer
Publication
- M. Zdrowowicz
- P. Wityk
- B. Michalska
- J. Rak
- ORGANIC & BIOMOLECULAR CHEMISTRY - Year 2016
A double-stranded oligonucleotide, 80 base pairs in length, was multiply labeled with 5-bromo-2′-deoxycytidine (BrdC) using polymerase chain reaction (PCR). The modified oligonucleotide was irradiated with 300 nm photons and its damage was assayed by employing DHPLC, LC-MS and denaturing polyacrylamide gel electrophoresis (PAGE). Two types of damage were demonstrated, namely, single strand breaks (SSBs) and intrastrand cross-links...

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