Construction, production and evaluation of the diagnostic utility of a recombinant Toxoplasma gondii chimeric antigen MIC1-MAG1-AMA1 - Open Research Data - Bridge of Knowledge

Your account

login

Search

Construction, production and evaluation of the diagnostic utility of a recombinant Toxoplasma gondii chimeric antigen MIC1-MAG1-AMA1

wersja 1.0

Description

The intracellular parasite Toxoplasma gondii has the ability to infect a wide range of warm-blooded animals, including humans. Currently, diagnosis of toxoplasmosis is based mainly on the use of the native antigens in enzyme immunoassay which allow for detection of IgG, IgM and IgA antibody classes. However, in some cases the performed studies give the ambiguous results. Moreover, the commercially available diagnostic tests due to its price are not generally applicable in the serodiagnosis of toxoplasmosis in animals, which requires the analysis of a large number of samples. For this reason, many research groups are currently working on new diagnostic tools, which are mainly recombinant antigens. Compared to the native antigens their production is much easier, cheaper, faster and safer. An additional advantage of the recombinant antigens is easier way to standardize assays.
This study presents the construction of an efficient Escherichia coli expression system for the production of recombinant chimeric protein composed of the immunodominant regions of three Toxoplasma gondii antigens MIC1, MAG1 and AMA1. The produced and purified proteins are composed of amino acid residues from:  A) 25-182 of the MIC1 antigen, 30-452 of the MAG1 antigen, 67-483 of the AMA1 antigen; B) 25-456 of the MIC1 antigen, 30-452 of the MAG1 antigen, 67-483 of the AMA1 antigen; C) 25-456 of the MIC1 antigen, 30-222 of the MAG1 antigen, 67-483 of the AMA1 antigen; D) 25-182 of the MIC1 antigen, 30-222 of the MAG1 antigen, 67-483 of the AMA1 antigen. In the next part of the work the diagnostic usefulness of obtaining proteins for detection of anti-T. gondii antibodies were evaluated in the IgG ELISA assay and agglutination test using animal sera (e.g. horses, ovine, and pigs) and human serum samples.

Dataset file

pET30 MIC1-MAG1-AMA1 II.zip
137.1 kB, S3 ETag 4561d86ed5d3e301f744709187ad3e1c-1, downloads: 2
The file hash is calculated from the formula
hexmd5(md5(part1)+md5(part2)+...)-{parts_count} where a single part of the file is 512 MB in size.

Example script for calculation:
https://github.com/antespi/s3md5
request access

File details

License:
Restricted access
This dataset is a part of the patent application. If you are interested, please contact me (bartlomiej.ferra@pg.edu.pl).
Software:
SnapGene Viewer

Details

Year of publication:
2021
Verification date:
2021-03-31
Creation date:
2021
Dataset language:
English
Fields of science:
  • biological sciences (Natural sciences)
  • health sciences (Medical and Health Sciences )
  • Veterinary science (Agricultural sciences)
DOI:
DOI ID 10.34808/txf2-0a91 open in new tab
Funding:
Ethical papers:
The sera were collected as part of the project entitled "Toxoplasmosis—facts and myths. Educational initiative raising social awareness about the infection with protozoan Toxoplasma gondii", foundation "Our Children", funded from the Civil Initiatives Fund of the Ministry of Labor and Social Policy (FIO 2008, contract No. 813).
Verified by:
Gdańsk University of Technology

Keywords

Cite as

Authors

Version this document has several versions

DOI 10.34808/vbcw-r854 represents the latest version of the data.

seen 89 times