Detection of UPEC IH11128 ability to form biofilm in a standard congo red (CR) method of colony staining.

Description

A standard method of testing E. coli's ability to form biofilm is based on the analysis of morphology
of bacterial colonies grown on yeast extract/casamino acids (YESCA) nutrient agar medium containing congo
red (CR) as an indicator dye of ECM production. The CR dye adsorbed from the culture medium by
bacteria binds to the components of the ECM, including curli and cellulose, giving the bacterial colonies a
dark red color.

The dataset contains original photos of bacterial colonies of the following E. coli strains: IH11128, DR14, CFT073, AAEC191A/pCC90 and AAEC191A/pACYC184. Bacteria were cultured on a YESCA medium at 25 degrees Celsius for 5 days. Pictures of the colonies were taken at intervals of one day.

Dataset file

Congo Red-experiment.zip

196.9 MB, S3 ETag d6e5bd0540974cb2d2c128b0984e8fca-1, downloads: 71

The file hash is calculated from the formula
hexmd5(md5(part1)+md5(part2)+...)-{parts_count} where a single part of the file is 512 MB in size.

Example script for calculation:
https://github.com/antespi/s3md5

download

File details

License:: open in new tab

CC 0

Public Domain Dedication
Raw data:: Data contained in dataset was not processed.

Details

Year of publication:

2023

Verification date:

2023-03-16

Creation date:

2022

Dataset language:

English

Fields of science:

biological sciences (Natural sciences)
chemical sciences (Natural sciences)

DOI:

10.34808/qh3q-4m93

Funding:

Mechanism of biofilm formation by uropatogenic E. coli producing fimbrial adhesin Dr - studies of proteome and metabolome changes under static and dynamic culture conditions

Verified by:

Gdańsk University of Technology

Keywords

Cite as

Authors

seen 144 times

Search