Determination of cytotoxic activity of new bisacridines IKE18, IKE19, IKE21 and IE10 against human kidney HEK-293 (ATCC® CRL-1573™) and liver cells HEPG2 (ATCC® HB-8065™)

Description

The datasets contain the results of determining in vitro cytotoxic activity of compounds using human cell lines assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, a method that evaluates cell viability by measuring cellular oxidoreductase activity. Initially, cells were seeded in 96-well culture plates and allowed to adhere overnight. The investigated compounds were added to the wells at various concentrations. Following a 72-hour incubation period, 20 µL of a 4 mg/mL MTT solution in PBS was added to each well, and the plates were further incubated for 3 hours under standard culture conditions. Subsequently, the culture medium was aspirated, and the formazan crystals were dissolved in DMSO. The absorbance was measured at 570 nm using a microplate reader (Asys UVM 340 Microplate Reader, Biochrom, Cambridge, UK).

Dataset file

Cyto IKE18, IKE19, IKE21, IE10.zip

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CC BY-NC-SA

Non-commercial - Share-alike
Raw data:: Data contained in dataset was not processed.
Software:: Excel

Details

Year of publication:

2023

Verification date:

2023-09-28

Dataset language:

English

Fields of science:

chemical sciences (Natural sciences)

DOI:

10.34808/agc2-7t26

Funding:

DNA topoisomerase inhibitors with acridine and acridone scaffold as a novel antifungal agents

Series:

Bisacridines

Verified by:

Gdańsk University of Technology

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