Toxoplasma godnii recombinant chimeric antigens - IgM and IgG ELISAs - mouse serum samples - Open Research Data - Bridge of Knowledge

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Toxoplasma godnii recombinant chimeric antigens - IgM and IgG ELISAs - mouse serum samples

Description

This study presents an evaluation of tetravalent recombinant chimeric proteins containing fragments of the Toxoplasma gondii antigens, SAG2, SAG1, GRA1, GRA2, MIC1, MAG1, ROP1 and AMA1, as potential replacements of a the soluble, whole-cell tachyzoite lysate (TLA) used in serological assays. Recombinant chimeric proteins (SAG1-MIC1-MAG1-GRA2, SAG2-GRA1-ROP1-GRA2, SAG2-GRA1-ROP1-AMA1N, AMA1N-SAG2-GRA1-ROP1, AMA1C-SAG2-GRA1-ROP1, and AMA1-SAG2-GRA1-ROP1) obtained by genetic engineering were tested for their reactivity with specific IgM and IgG antibodies from sera of experimentally infected mice and humans with T. gondii infection using an enzyme-linked immunosorbent assay (ELISA). Serum samples from patients with acquired T. gondii infection and sera from seronegative individuals were examined. The reactivity of chimeric antigens with antibodies generated during T. gondii invasion was measured and compared to the results obtained in assays based on whole-cell Toxoplasma antigen. Chimeric proteins proved effective in differentiation between T. gondii-infected and uninfected individuals (100% sensitivity and specificity in the IgG ELISAs) which shows their potential usefulness as a replacements for TLA in standardized commercial tests for the serodiagnosis of toxoplasmosis

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MOST DANYCH - MOUSE RESULTS.xlsx
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License:
Creative Commons: by 4.0 open in new tab
CC BY
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Details

Year of publication:
2019
Verification date:
2020-12-17
Creation date:
2018
Dataset language:
English
Fields of science:
  • biological sciences (Natural sciences)
  • medical sciences (Medical and Health Sciences )
DOI:
DOI ID 10.34808/bw9j-yq04 open in new tab
Funding:
Ethical papers:
All experimental procedures were carried out according to guidelines provided by the Polish Local Ethics Commission for Experiments on Animals No. 9 in Lodz (Agreement 75/ŁB639/2012).
Verified by:
Gdańsk University of Technology

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