Electrochemical Au-Minecraft: a new approach towards the impedimetric biosensing system

The main task of the project is to examine different forms of interaction and self-organization of gold nanoparticles with the surface of the electrode under the influence of the electric field. The generated heterogeneous distribution of diffusion fields will affect the global behavior of the electrode, and therefore the effect of self-organization will be a specific amplification of the impedance response that is detected during the operation of the biosensor. The proposed solution will translate into an increase in the sensitivity and selectivity of macromolecular compounds detection, and will also offer a new range of solutions dedicated to biosensor functionalization. The project will contribute to the development of the laboratory to study electrochemical and physicochemical phenomena of the Gdańsk University of Technology, resulting in numerous publications in Q1 journals. New experimental tools and modeling procedures will be developed. The project closely matches the topics implemented within Advanced Materials and BioTechMed Centres, contributing the development of GUT's main research areas as part of the IDUB project. The work will be conducted in close cooperation with researchers of the University of Gdańsk: Faculties of Chemistry and Biology.

Details

Project's acronym:

AUMEIS

Financial Program Name:

OPUS

Organization:

Narodowe Centrum Nauki (NCN) (National Science Centre)

Agreement:

UMO-2020/37/B/ST7/03262 z dnia 2021-01-26

Realisation period:

2021-01-26 - 2025-01-25

Project manager:

dr hab. inż. Jacek Ryl

Team members:

Researcher dr hab. inż. Robert Bogdanowicz
Scholarship grantee/Student/PhD student Adrian Olejnik

Realised in:

Institute of Nanotechnology and Materials Engineering

External institutions participating in project:

Uniwersytet Gdański (Poland)

Project's value:

1 276 200.00 PLN

Request type:

National Research Programmes

Domestic:

Domestic project

Verified by:

Gdańsk University of Technology

Papers associated with that project

results on page:
year:
- selected sort by year newest first
- sort by year oldest first
title:
- selected sort by title A-Z
- sort by title Z-A
citation:
- sort by citation descending
- sort by citation ascending

Filters

total: 6

Year 2024

Electrochemical detection of bacterial endotoxin lipopolysaccharide (LPS) on gold electrode modified with DAL-PEG-DK5-PEG-OH - Antimicrobial peptide conjugate
Publication
- K. Paulina
- A. Golda
- J. Ryl
- M. Pilarczyk-zurek
- G. Bereta
- T. Ossowski
- A. Lesner
- J. Koziel
- A. Prahl
- P. Niedziałkowski
- TALANTA.The International Journal of Pure and Applied Analytical Chemistry - Year 2024
This work describes fabrication of gold electrodes modified with peptide conjugate DAL-PEG-DK5-PEG-OH that enables ultra-sensitive detection of lipopolysaccharide (LPS) isolated from the reference strain of Escherichia coli O26:B6. The initial step of the established procedure implies immobilization of the fully protected DAL-PEG-DK5-PEG-OH peptide on the surface of the gold electrode previously modified by cysteamine. Then side...

Full text to download in external service

Year 2023

An electrochemical biosensor for the determination of hormone Human Chorionic Gonadotropin (hCG) in human serum
Publication
- A. Koterwa
- M. Bojko
- J. Ryl
- K. Łukaszuk
- K. Kozłowska
- W. Sieklicki
- S. Rodziewicz-Motowidło
- P. Niedzialkowski
- ELECTROANALYSIS - Year 2023
This work describes the modification of a gold electrode to create an electrochemical biosensor capable of detecting human chorionic gonadotropin (hCG). The biosensor was obtained by modifying the gold electrode with cysteamine and oligopeptide (PPLRINRHILTR). The modification steps of the gold electrode were confirmed by cyclic voltammetry (CV) and impedance electrochemical spectroscopy (EIS) measurements. The conducted EIS experiments...

Full text available to download
Discriminating macromolecular interactions based on an impedimetric fingerprint supported by multivariate data analysis for rapid and label-free Escherichia coli recognition in human urine
Publication
- A. Koterwa
- M. Pierpaoli
- B. Nejman-Faleńczyk
- S. Bloch
- A. Zieliński
- W. Adamus-Białek
- Z. Jeleniewska
- B. Trzaskowski
- R. Bogdanowicz
- G. Węgrzyn... and 2 others
- BIOSENSORS & BIOELECTRONICS - Year 2023
This manuscript presents a novel approach to address the challenges of electrode fouling and highly complex electrode nanoarchitecture, which are primary concerns for biosensors operating in real environments. The proposed approach utilizes multiparametric impedance discriminant analysis (MIDA) to obtain a fingerprint of the macromolecular interactions on flat glassy carbon surfaces, achieved through self-organized, drop-cast,...

Full text available to download
Texture or Linker? Competitive Patterning of Receptor Assembly toward Ultra-Sensitive Impedimetric Detection of Viral Species at Gold-Nanotextured Titanium Surfaces
Publication
- W. Lipińska
- A. Olejnik
- M. Janik
- M. Brodowski
- K. Sapiega
- M. Pierpaoli
- K. Siuzdak
- R. Bogdanowicz
- J. Ryl
- Journal of Physical Chemistry C - Year 2023
In this work, we study the electrodes with a periodic matrix of gold particles pattered by titanium dimples and modified by 3-mercaptopropionic acid (MPA) followed by CD147 receptor grafting for specific impedimetric detection of SARS-CoV-2 viral spike proteins. The synergistic DFT and MM/MD modeling revealed that MPA adsorption geometries on the Au–Ti surface have preferential and stronger binding patterns through the carboxyl...

Full text available to download

Year 2022

Deciphering the Molecular Mechanism of Substrate-Induced Assembly of Gold Nanocube Arrays toward an Accelerated Electrocatalytic Effect Employing Heterogeneous Diffusion Field Confinement
Publication
- P. Niedziałkowski
- A. Koterwa
- A. Olejnik
- A. Zieliński
- K. Górnicka
- M. Brodowski
- R. Bogdanowicz
- J. Ryl
- LANGMUIR - Year 2022
The complex electrocatalytic performance of gold nanocubes (AuNCs) is the focus of this work. The faceted shapes of AuNCs and the individual assembly processes at the electrode surfaces define the heterogeneous conditions for the purpose of electrocatalytic processes. Topographic and electron imaging demonstrated slightly rounded AuNC (average of 38 nm) assemblies with sizes of ≤1 μm, where the dominating patterns are (111) and...

Full text available to download
Enhanced susceptibility of SARS-CoV-2 spike RBD protein assay targeted by cellular receptors ACE2 and CD147: Multivariate data analysis of multisine impedimetric response
Publication
- M. Brodowski
- M. Pierpaoli
- M. Janik
- M. Kowalski
- M. Ficek
- P. Ślepski
- B. Trzaskowski
- G. N. Swain
- J. Ryl
- R. Bogdanowicz
- SENSORS AND ACTUATORS B-CHEMICAL - Year 2022
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) enters the cells through the binding of spike protein to the host cell surface-expressing angiotensin-converting enzyme 2 (ACE2) or by endocytosis mediated by extracellular matrix metalloproteinase inducer (CD147). We present extended statistical studies of the multisine dynamic electrochemical impedance spectroscopy (DEIS) revealing interactions between Spike RBD and...

Full text available to download

seen 2014 times