A comparative analysis of phenotypic and genotypic methods for the determination of the biofilm-forming abilities of Staphylococcus epidermidis - Publication - Bridge of Knowledge

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A comparative analysis of phenotypic and genotypic methods for the determination of the biofilm-forming abilities of Staphylococcus epidermidis

Abstract

The collection of 146 Staphylococcus epidermidis strains isolated from the nasopharynx of lung cancer patients has been studied for the ability of slime secretion and biofilm formation using the Congo red agar (CRA) test and the microtiter plate (MtP) method, respectively. The prevalence of the icaAD and the aap genes was also analyzed. Some isolates (35.6%) were biofilm positive by the MtP method, while 58.9% of isolates exhibited a slime-positive phenotype by the CRA test. The sensitivities of the CRA test evaluated using the MtP method as a gold standard of biofilm production were 73.1%, 97.3% and 13.3% for all the strains screened, ica-positive and ica-negative strains, respectively. The genotype ica(+)aap(+) was correlated with a strong biofilm-producer phenotype. Interestingly, some of the ica(-)aap(-) isolates could also form a biofilm. The correlation between the presence of icaAD genes and the biofilm-positive phenotype by the MtP method as well as slime production by the CRA test was statistically significant (P<0.0001). However, some S. epidermidis strains possess the potential ability of ica-independent biofilm formation; thus, further studies are needed to determine reliable, short-time criteria for an in vitro assessment of biofilm production by staphylococci.

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Authors (8)

  • Photo of  Renata Los

    Renata Los

  • Photo of  Rafał Sawicki

    Rafał Sawicki

  • Photo of  Marek Juda

    Marek Juda

  • Photo of  Magdalena Stankevic

    Magdalena Stankevic

  • Photo of  Paweł Rybojad

    Paweł Rybojad

  • Photo of  Marek Sawicki

    Marek Sawicki

  • Photo of  Anna Malm

    Anna Malm

  • Photo of  Grażyna Ginalska

    Grażyna Ginalska

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Category:
Magazine publication
Type:
Magazine publication
Published in:
FEMS MICROBIOLOGY LETTERS no. 310, edition 2, pages 97 - 103,
ISSN: 0378-1097
Publication year:
2010
Bibliographic description:
Los R., Sawicki R., Juda M., Stankevic M., Rybojad P., Sawicki M., Malm A., Ginalska G.: A comparative analysis of phenotypic and genotypic methods for the determination of the biofilm-forming abilities of Staphylococcus epidermidis// FEMS MICROBIOLOGY LETTERS -Vol. 310,iss. 2 (2010), s.97-103
DOI:
Digital Object Identifier (open in new tab) 10.1111/j.1574-6968.2010.02050.x
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