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Quantitative Analysis of Biofilm Formed on Vascular Prostheses by Staphylococcus Epidermidis with Different ica and aap Genetic Status
Abstract
OBJECTIVES: This study aims to examine biofilm formed on vascular prostheses by Staphylococcus epidermidis with different ica and aap genetic status, and to evaluate the effect of antibiotic-modified prostheses on bacterial colonization. METHODS: Biofilm formation was determined using fluorescence microscopy imaging. Quantitative analysis was conducted using the biofilm coverage ratio (BCR) calculations. RESULTS: Our investigations prove that the BCR method with fluorescent dye enabled an accurate assessment of biofilm coverage and comparison of the obtained results. The ica+ aap+ strains formed a biofilm on all of the examined vascular prostheses. Uni-Graft(®) modified with covalently immobilized amikacin was effective in preventing bacterial adherence. CONCLUSIONS: Molecular biology techniques combined with phenotype studies give a broad insight into biofilm formation mechanisms. On the other hand, fluorescence microscopy imaging along with BCR calculations are reliable and simple tools to quantitatively estimate biofilm formation, as well as the effectiveness of antimicrobial prosthesis modification.
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Authors (6)
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Joanna Golus
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Rafal Sawicki
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Renata Los
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Anna Malm
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Grazyna Ginalska
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Details
- Category:
- Articles
- Type:
- artykuły w czasopismach
- Published in:
-
INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS
no. 36,
pages 105 - 112,
ISSN: 0391-3988 - Language:
- English
- Publication year:
- 2013
- Bibliographic description:
- Golus J., Stankevic M., Sawicki R., Los R., Malm A., Ginalska G.: Quantitative Analysis of Biofilm Formed on Vascular Prostheses by Staphylococcus Epidermidis with Different ica and aap Genetic Status// INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS -Vol. 36,iss. 2 (2013), s.105-112
- DOI:
- Digital Object Identifier (open in new tab) 10.5301/ijao.5000157
- Verified by:
- Gdańsk University of Technology
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