Search
A novel method of Mycobacterium tuberculosis complex strain differentiation using polymorphic GC-rich gene sequences.
Abstract
Tuberculosis is one of the leading infectious diseases. In this work, a new genotyping method of Mycobacterium tuberculosis (Mtb) complex strain is presented. 27 Mtb genomes were analyzed for the presence of length polymorphism within polymorphic GC-rich gene sequences. Four genes, Rv3345c, Rv3507, Rv0747 and Rv3511, showing variation in length depending on the Mtb strain were selected for designing primer sequences flanking variable regions for the PCR method. Identification of 16 genotypes among 27 analyzed genomes demonstrated usefulness of our genotyping method in differentiation of Mtb genomes based on sequence polymorphism in the four PGRS genes.
Citations
-
3
CrossRef
-
0
Web of Science
-
3
Scopus
Cite as
Full text
full text is not available in portal
Keywords
Details
- Category:
- Other
- Type:
- supllement, wydanie specjalne, dodatek
- Published in:
-
Acta Biochimica Polonica
no. 62,
pages 317 - 322,
ISSN: 0001-527X - Language:
- English
- Publication year:
- 2015
- DOI:
- Digital Object Identifier (open in new tab) 10.18388/abp.2015_1037
- Verified by:
- Gdańsk University of Technology
seen 132 times
Recommended for you
PCR-Based Genotyping of Mycobacterium tuberculosis with New GC-Rich Repeated Sequences and IS6110 Inverted Repeats Used as Primers.
- R. Kotłowski,
- I. C. Shamputa,
- N. A. El Aila
- + 4 authors
2004
Molecular characterization of drug-resistant Mycobacterium tuberculosis strains from Poland by use of multiple genetic markers
- A. Sajduda,
- J. Dziadek,
- R. Kotłowski
- + 1 authors
2006