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Antimicrobial Activity of Chimera Peptides Composed of Human Neutrophil Peptide 1 (HNP-1) Truncated Analogues and Bovine Lactoferrampin
Abstract
Three chimera peptides composed of bovine lactoferrampin and the analogue of truncated human neutrophil peptide 1 were synthesized by the solid-phase method. In two compounds peptide chains were connected via isopeptide bond, whereas in the third one disulfide bridge served as a linker. All three chimeras displayed significantly higher antimicrobial activity than the constituent peptides as well as their equimolar mixtures. The one with a disulfide bridge displayed selectivity toward Gram-positive bacteria and was able to penetrate bacterial cells. The chimeric peptides demonstrated low in vitro mammalian cytotoxicity, especially against benign cells. The significance of linker type was also reflected in the secondary structure and proteolytic stability of studied compounds. Presented results proved that such chimeras are good lead structures for designing antimicrobial drugs.
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Authors (12)
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Natalia Ptaszyńska
- Uniwersytet Gdański, Wydział Chemii Katedra Biochemii Molekularnej
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Anna Łęgowska
- Uniwersytet Gdański, Wydział Chemii Katedra Biochemii Molekularnej
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Dawid Dębowski
- Uniwersytet Gdański, Wydział Chemii Katedra Biochemii Molekularnej
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Agata Gitlin-Domagalska
- Uniwersytet Gdański, Wydział Chemii Katedra Biochemii Molekularnej
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Tzi Ng
- The Chinese University of Hong Kong, Faculty of Medicine School of Biomedical Sciences
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Krzysztof Rolka
- Uniwersytet Gdański, Wydział Chemii Katedra Biochemii Molekularnej
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Details
- Category:
- Articles
- Type:
- artykuł w czasopiśmie wyróżnionym w JCR
- Published in:
-
BIOCONJUGATE CHEMISTRY
no. 29,
edition 9,
pages 3060 - 3071,
ISSN: 1043-1802 - Language:
- English
- Publication year:
- 2018
- Bibliographic description:
- Ptaszyńska N., Gucwa K., Łęgowska A., Dębowski D., Gitlin-Domagalska A., Lica J., Heldt M., Martynow D., Olszewski M., Milewski S., Ng T., Rolka K.: Antimicrobial Activity of Chimera Peptides Composed of Human Neutrophil Peptide 1 (HNP-1) Truncated Analogues and Bovine Lactoferrampin// BIOCONJUGATE CHEMISTRY. -Vol. 29, iss. 9 (2018), s.3060-3071
- DOI:
- Digital Object Identifier (open in new tab) 10.1021/acs.bioconjchem.8b00440
- Verified by:
- Gdańsk University of Technology
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