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Inhibitory and antimicrobial activities of OGTI and HV-BBI peptides, fragments and analogs derived from amphibian skin
Abstract
A series of linear and cyclic fragments and analogs of two peptides (OGTI and HV-BBI) isolated from skin secretions of frogs were synthesized by the solid-phase method. Their inhibitory activity against several serine proteinases: bovine beta-trypsin, bovine alpha-chymotypsin, human leukocyte elastase and cathepsin G from human neutrophils, was investigated together with evaluation of their antimicrobial activities against Gram-negative bacteria (Escherichia coli) and Gram-positive species isolated from patients (Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus sp., Streptococcus sp.). The cytotoxicity of the selected peptides toward an immortal human skin fibroblast cell line was also determined. Three peptides: HV-BBI, its truncated fragment HV-BBI(3-18) and its analog [Phe(8)]HV-BBI can be considered as bifunctional compounds with inhibitory as well as antibacterial properties. OGTI, although it did not display trypsin inhibitory activity as previously reported in the literature, exerted antimicrobial activity toward S. epidermidis. In addition, under our experimental conditions, this peptide did not show cytotoxicity. (c) 2012 Elsevier Inc. All rights reserved.
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Authors (10)
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Dawid Dębowski
- Wydział Chemii UG Katedra Chemii Bioorganicznej
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Anna Łęgowska
- Wydział Chemii UG Katedra Chemii Bioorganicznej
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Natalia Karna
- Wydział Chemii UG Katedra Chemii Bioorganicznej
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Michał Pikuła
- Gdański Uniwersytet Medyczny Zakład Imunologi Klinicznej i Transplantologii
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Magdalena Wysocka
- Wydział Chemii UG Katedra Chemii Bioorganicznej
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Irena Maliszewska
- Wydział Chemii UG Katedra Chemii Bioorganicznej
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Marcin Sieńczyk
- Wydział Chemiczny PW Zakład Chemii Medycznej i Mikrobiologii
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Krzysztof Rolka
- Wydział Chemii UG Katedra Chemii Bioorganicznej
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Keywords
Details
- Category:
- Articles
- Type:
- artykuł w czasopiśmie wyróżnionym w JCR
- Published in:
-
PEPTIDES
no. 35,
edition 2,
pages 276 - 284,
ISSN: 0196-9781 - Language:
- English
- Publication year:
- 2012
- Bibliographic description:
- Dębowski D., Łukajtis R., Łęgowska A., Karna N., Pikuła M., Wysocka M., Maliszewska I., Sieńczyk M., Lesner A., Rolka K.: Inhibitory and antimicrobial activities of OGTI and HV-BBI peptides, fragments and analogs derived from amphibian skin// PEPTIDES. -Vol. 35, iss. 2 (2012), s.276-284
- DOI:
- Digital Object Identifier (open in new tab) 10.1016/j.peptides.2012.04.001
- Verified by:
- Gdańsk University of Technology
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