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CaZnO-based nanoghosts for the detection of ssDNA, pCRISPR and recombinant SARS-CoV-2 spike antigen and targeted delivery of doxorubicin
Abstract
Overexpression of proteins/antigens and other gene-related sequences in the bodies could lead to significant mutations and refractory diseases. Detection and identification of assorted trace concentrations of such proteins/antigens and/or gene-related sequences remain challenging, affecting different pathogens and making viruses stronger. Correspondingly, coronavirus (SARS-CoV-2) mutations/alterations and spread could lead to overexpression of ssDNA and the related antigens in the population and brisk activity in gene-editing technologies in the treatment/detection may lead to the presence of pCRISPR in the blood. Therefore, the detection and evaluation of their trace concentrations are of critical importance. CaZnO-based nanoghosts (NGs) were synthesized with the assistance of a high-gravity technique at a 1,800 MHz field, capitalizing on the use of Rosmarinus officinalis leaf extract as the templating agent. A complete chemical, physical and biological investigation revealed that the synthesized NGs presented similar morphological features to the mesenchymal stem cells (MSCs), resulting in excellent biocompatibility, interaction with ssDNA- and/or pCRISPR-surface, through various chemical and physical mechanisms. This comprise the unprecedented synthesis of a fully inorganic nanostructure with behavior that is similar to MSCs. Furthermore, the endowed exceptional ability of inorganic NGs for detective sensing/folding of ssDNA and pCRISPR and recombinant SARS-CoV-2 spike antigen (RSCSA), along with in-situ hydrogen peroxide detection on the HEK-293 and HeLa cell lines, was discerned. On average, they displayed a high drug loading capacity of 55%, and the acceptable internalizations inside the HT-29 cell lines affirmed the anticipated MSCs-like behavior of these inorganic-NGs.
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Authors (14)
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Navid Rabiee
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Omid Akhavan
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Yousef Fatahi
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Amir Mohammad Ghadiri
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Mahsa Kiani
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Pooyan Makvandi
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Mohammad Rabiee
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Mohammad Hossein Nicknam
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Rajender S. Varma
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Milad Ashrafizadeh
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Ehsan Nazarzadeh Zare
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Esmaeel Sharifi
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Eder C. Lima
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Keywords
Details
- Category:
- Articles
- Type:
- artykuły w czasopismach
- Published in:
-
CHEMOSPHERE
no. 306,
ISSN: 0045-6535 - Language:
- English
- Publication year:
- 2022
- Bibliographic description:
- Rabiee N., Akhavan O., Fatahi Y., Ghadiri A. M., Kiani M., Makvandi P., Rabiee M., Nicknam M. H., Saeb M., Varma R. S., Ashrafizadeh M., Zare E. N., Sharifi E., Lima E. C.: CaZnO-based nanoghosts for the detection of ssDNA, pCRISPR and recombinant SARS-CoV-2 spike antigen and targeted delivery of doxorubicin// CHEMOSPHERE -Vol. 306, (2022), s.135578-
- DOI:
- Digital Object Identifier (open in new tab) 10.1016/j.chemosphere.2022.135578
- Sources of funding:
-
- Free publication
- Verified by:
- Gdańsk University of Technology
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