Chitosan-protein scaffolds loaded with lysostaphin as potential antistaphylococcal wound dressing materials. - Publication - Bridge of Knowledge

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Chitosan-protein scaffolds loaded with lysostaphin as potential antistaphylococcal wound dressing materials.

Abstract

AIMS: The development of technology for preparing chitosan-protein scaffolds loaded with lysostaphin, which potentially could be used as dressing for wound treatment and soft tissue infections caused by Staphylococcus aureus. METHODS AND RESULTS: The unique technology of chitosan solubilization using gaseous CO(2) instead of organic or inorganic acids was used for the incorporation of lysostaphin, the enzyme that exhibits bactericidal activity against staphylococci, within the structure of chitosan-protein sponges. The developed chitosan-protein scaffolds loaded with lysostaphin revealed high antistaphylococcal activity, which has been confirmed with a large (n = 143) collection of clinical (skin and wound infections) and animal (bovine mastitis) isolates of these bacteria, including MRSA. No change of bactericidal activity of the lyophilized materials has been observed during half-year storage at 4°C. CONCLUSIONS: The developed materials are potential candidates for preparing biologically active, antistaphylococcal wound dressing materials. SIGNIFICANCE AND IMPACT OF THE STUDY: Staphylococci belong to the most popular and most burdensome aetiological factors of wound and soft tissues infections. The developed chitosan-protein scaffolds loaded with lysostaphin could be a possible solution to problems associated with treatment of these infections.

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Category:
Articles
Type:
artykuł w czasopiśmie wyróżnionym w JCR
Published in:
JOURNAL OF APPLIED MICROBIOLOGY no. 117, edition 3, pages 634 - 642,
ISSN: 1364-5072
Language:
English
Publication year:
2014
Bibliographic description:
Szweda P., Gorczyca G., Tylingo R., Kurlenda J., Kwieciński J., Milewski S.: Chitosan-protein scaffolds loaded with lysostaphin as potential antistaphylococcal wound dressing materials.// JOURNAL OF APPLIED MICROBIOLOGY. -Vol. 117, iss. 3 (2014), s.634-642
DOI:
Digital Object Identifier (open in new tab) 10.1111/jam.12568
Verified by:
Gdańsk University of Technology

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