Chitosan-protein scaffolds loaded with lysostaphin as potential antistaphylococcal wound dressing materials.

Piotr Szweda; Grzegorz Gorczyca; Robert Tylingo; Julianna Kurlenda; J. Kwieciński; Sławomir Milewski

doi:10.1111/jam.12568

Chitosan-protein scaffolds loaded with lysostaphin as potential antistaphylococcal wound dressing materials.

Abstrakt

AIMS: The development of technology for preparing chitosan-protein scaffolds loaded with lysostaphin, which potentially could be used as dressing for wound treatment and soft tissue infections caused by Staphylococcus aureus. METHODS AND RESULTS: The unique technology of chitosan solubilization using gaseous CO(2) instead of organic or inorganic acids was used for the incorporation of lysostaphin, the enzyme that exhibits bactericidal activity against staphylococci, within the structure of chitosan-protein sponges. The developed chitosan-protein scaffolds loaded with lysostaphin revealed high antistaphylococcal activity, which has been confirmed with a large (n = 143) collection of clinical (skin and wound infections) and animal (bovine mastitis) isolates of these bacteria, including MRSA. No change of bactericidal activity of the lyophilized materials has been observed during half-year storage at 4°C. CONCLUSIONS: The developed materials are potential candidates for preparing biologically active, antistaphylococcal wound dressing materials. SIGNIFICANCE AND IMPACT OF THE STUDY: Staphylococci belong to the most popular and most burdensome aetiological factors of wound and soft tissues infections. The developed chitosan-protein scaffolds loaded with lysostaphin could be a possible solution to problems associated with treatment of these infections.

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Autorzy (6)

Piotr Szweda dr hab. inż.
Grzegorz Gorczyca mgr inż.
Robert Tylingo dr hab. inż.
Julianna Kurlenda
- tate Higher Vocational School in Koszalin tate Higher Vocational School in Koszalin
J. Kwieciński
- Sahlgrenska Academy at University of Gothenburg Department of Rheumatology and Inflammation Research
Sławomir Milewski prof. dr hab. inż.

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Kategoria:: Publikacja w czasopiśmie
Typ:: artykuł w czasopiśmie wyróżnionym w JCR
Opublikowano w:: JOURNAL OF APPLIED MICROBIOLOGY nr 117, wydanie 3, strony 634 - 642,
ISSN: 1364-5072
Język:: angielski
Rok wydania:: 2014
Opis bibliograficzny:: Szweda P., Gorczyca G., Tylingo R., Kurlenda J., Kwieciński J., Milewski S.: Chitosan-protein scaffolds loaded with lysostaphin as potential antistaphylococcal wound dressing materials.// JOURNAL OF APPLIED MICROBIOLOGY. -Vol. 117, iss. 3 (2014), s.634-642
DOI:: Cyfrowy identyfikator dokumentu elektronicznego (otwiera się w nowej karcie) 10.1111/jam.12568
Weryfikacja:: Politechnika Gdańska