DMSO and TMAO—Differences in Interactions in Aqueous Solutions of the K-Peptide - Publication - Bridge of Knowledge

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DMSO and TMAO—Differences in Interactions in Aqueous Solutions of the K-Peptide

Abstract

Interactions between a solvent and their co-solute molecules in solutions of peptides are crucial for their stability and structure. The K-peptide is a synthetic fragment of a larger hen egg white lysozyme protein that is believed to be able to aggregate into amyloid structures. In this study, a complex experimental and theoretical approach is applied to study systems comprising the peptide, water, and two co-solutes: trimethylamide N-oxide (TMAO) or dimethyl sulfoxide (DMSO). Information about their interactions in solutions and on the stability of the K-peptide was obtained by FTIR spectroscopy and differential scanning microcalorimetry. The IR spectra of various osmolyte–water–model-peptide complexes were simulated with the DFT method (B3LYP/6-311++G(d,p)). The FTIR results indicate that both solutes are neutral for the K-peptide in solution. Both co-solutes affect the peptide to different degrees, as seen in the shape of its amide I band, and have different influences on its thermal stability. DFT calculations helped simplify the experimental data for easier interpretation.

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DOI:
Digital Object Identifier (open in new tab) 10.3390/ijms23031872
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Category:
Articles
Type:
artykuły w czasopismach
Published in:
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES no. 23,
ISSN: 1661-6596
Language:
English
Publication year:
2022
Bibliographic description:
Godlewska J., Cieśla B., Wawer J., Bruździak P.: DMSO and TMAO—Differences in Interactions in Aqueous Solutions of the K-Peptide// INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES -Vol. 23,iss. 3 (2022), s.1872-
DOI:
Digital Object Identifier (open in new tab) 10.3390/ijms23031872
Sources of funding:
Verified by:
Gdańsk University of Technology

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