DNA Methylation Changes Induced by Redox-Active Compounds—Choosing the Right PCR-Based Method - Publication - Bridge of Knowledge

Search

DNA Methylation Changes Induced by Redox-Active Compounds—Choosing the Right PCR-Based Method

Abstract

The impact of catechins on the expression profile of redox-related genes in HT29 cell line has been studied recently by our group using Oxidative Stress RT2 Profiler PCR Array. Within the examined panel of 84 genes, the down-regulation of SRXN1 gene was unique among other up-regulated genes. We hypothesized that the observed down-regulation resulted from DNA methylation and have exploited this observation to choose the proper strategy to monitor the changes in DNA methylation patterns incurred by dietary antioxidants. The current study verified two PCR-based approaches.

Citations

  • 1

    CrossRef

  • 0

    Web of Science

  • 0

    Scopus

Cite as

Full text

download paper
downloaded 13 times
Publication version
Accepted or Published Version
License
Creative Commons: CC-BY open in new tab

Keywords

Details

Category:
Articles
Type:
publikacja w in. zagranicznym czasopiśmie naukowym (tylko język obcy)
Published in:
Proceedings no. 11, pages 1 - 4,
ISSN:
Language:
English
Publication year:
2019
Bibliographic description:
Jakubek P., Rajić J., Baranowska M., Vidaković M., Bartoszek-Pączkowska A., Namieśnik J.. DNA Methylation Changes Induced by Redox-Active Compounds—Choosing the Right PCR-Based Method. Proceedings, 2019, Vol. 11, iss. 1, s.1-4
DOI:
Digital Object Identifier (open in new tab) 10.3390/proceedings2019011020
Bibliography: test
  1. Akika. R.; Awada, Z.; Mogharbil, N.; Zgheib, N.K. Region of interest methylation analysis: A comparison of MSP with MS-HRM and direct BSP. Mol. Biol. Rep. 2017, 44, 295-305, doi:10.1007/s11033-017-4110-7. open in new tab
  2. Baranowska, M.; Suliborska, K.; Chrzanowski, W.; Kusznierewicz, B.; Namieśnik, J.; Bartoszek, A. The relationship between standard reduction potentials of catechins and biological activities involved in redox control. Redox Biol. 2018, 17, 355-366, doi:10.1016/j.redox.2018.05.005. open in new tab
  3. Hyun, A.W.; Jeong, W.; Chang, T.-S.; Park, K.J.; Park, S.J.; Yang, J.S.; Rhee, S.G. Reduction of cysteine sulfinic acid by sulfiredoxin is specific to 2-Cys peroxiredoxins. J. Biol. Chem. 2005, 280, 3125-3128, doi:10.1074/jbc.C400496200. open in new tab
  4. Wei, Q.; Jian, H.; Baker, A.; Dodge, L.K.; Gerard, M.; Young, M.R.; Toledano, M.B.; Colburn, N.H. Loss of sulfiredoxin renders mice resistant to azoxymethane/dextran sulfate sodiuminduced colon carcinogenesis. Carcinogenesis 2013, 34, 1403-1410, doi:10.1093/carcin/bgt059. open in new tab
  5. Wei, Q.; Jiang, H.; Matthews, C.P.; Colburn, N.H. Sulfiredoxin is an AP-1 target gene that is required for transformation and shows elevated expression in human skin malignancies. PNAS 2008, 105, 19738-19743, doi:10.1073/pnas.0810676105. open in new tab
  6. Wei, Q.; Jiang, H.; Xiao, Z.; Baker, A.; Young, M.R.; Veenstra, T.D.; Colburn, N.H. Sulfiredoxin-Peroxiredoxin IV axis promotes human lung cancer progression through modulation of specific phosphokinase signaling. PNAS 2011, 108, 7004-7009, doi:10.1073/pnas.1013012108. open in new tab
Sources of funding:
  • Cost Action "NutRedOx" CA16112
Verified by:
Gdańsk University of Technology

seen 148 times

Recommended for you

Meta Tags