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Evaluation of Three Peptide Immobilization Techniques on a QCM Surface Related to Acetaldehyde Responses in the Gas Phase
Abstract
The quartz-crystal microbalance is a sensitive and universal tool for measuring concentrations of various gases in the air. Biochemical functionalization of the QCM electrode allows a label-free detection of specific molecular interactions with high sensitivity and specificity. In addition, it enables a real-time determination of its kinetic rates and affinity constants. This makes QCM a versatile bioanalytical screening tool for various applications, with surface modifications ranging from the detection of single molecular monolayers to whole cells. Various types of biomaterials, including peptides mapping the binding sites of olfactory receptors, can be deposited as a sensitive element on the surface of the electrodes. One of key ways to ensure the sensitivity and accuracy of the sensor is provided by application of an optimal and repeatable method of immobilization. Therefore, effective sensors operation requires development of an optimal method of deposition. This paper reviews popular techniques (drop-casting, spin-coating, dip-coating) for coating peptides on piezoelectric crystals surface.
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Authors (5)
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Tomasz Wasilewski mgr
- Gdański Uniwersytet Medyczny
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Wojciech Kamysz dr hab
- Gdański Uniwersytet Medyczny
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Full text
- Publication version
- Accepted or Published Version
- DOI:
- Digital Object Identifier (open in new tab) 10.3390/s18113942
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Details
- Category:
- Articles
- Type:
- artykuł w czasopiśmie wyróżnionym w JCR
- Published in:
-
SENSORS
no. 18,
edition 11,
ISSN: 1424-8220 - Language:
- English
- Publication year:
- 2018
- Bibliographic description:
- Wasilewski T., Szulczyński B., Kamysz W., Gębicki J., Namieśnik J.: Evaluation of Three Peptide Immobilization Techniques on a QCM Surface Related to Acetaldehyde Responses in the Gas Phase// SENSORS. -Vol. 18, iss. 11 (2018), s.3942-
- DOI:
- Digital Object Identifier (open in new tab) 10.3390/s18113942
- Sources of funding:
-
- Free publication
- Verified by:
- Gdańsk University of Technology
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