Keywords

Details

Category:

Thesis, nostrification

Type:

praca doktorska pracowników zatrudnionych w PG oraz studentów studium doktoranckiego

Language:

Polish

Publication year:

2018

Bibliography: test

1. Aparatura i sprzęt laboratoryjny ................................................................................................... 44
2. Odczynniki chemiczne ........................................................................................................................ 45
3. Pożywki i podłoża mikrobiologiczne ........................................................................................... 46 open in new tab
4. Markery..................................................................................................................................................... 46
5. Odczynniki do reakcji PCR................................................................................................................ 47
6. Enzymy restrykcyjne i ich bufory do reakcji trawienia....................................................... 50
7. Odczynniki do reakcji Gibsona ....................................................................................................... 50
8. Odczynniki do rozdziału DNA za pomocą elektroforezy agarozowej ........................... 50 open in new tab
9. Odczynniki do rozdziału białek za pomocą elektroforezy poliakrylamidowej w warunkach denaturujących (SDS-PAGE) ................................................................................... 51
10. Bufory do oczyszczania białek ..................................................................................................... 52 12. Odczynniki do filtracji żelowej .................................................................................................... 53 13. Szczepy bakteryjne, DNA plazmidowe i genomowe .......................................................... 53 14. Odczynniki do izolacji genomowego DNA .............................................................................. 54 15. Odczynniki do izolacji plazmidowego DNA............................................................................ 54
11. Odczynniki do oczyszczania DNA z żelu agarozowego i po reakcjach trawienia enzymatycznego ........................................................................................................................................ 54 17. Zestaw do oznaczania jednostkowości polimeraz DNA ................................................... 54
12. Pozostałe bufory i roztwory ......................................................................................................... 55 METODY ................................................................................................................................................................. 56 1. Izolacja genomowego DNA ............................................................................................................... 56 2. Izolacja plazmidowego DNA ............................................................................................................ 56 3. Elektroforetyczny rozdział DNA w żelu agarozowym ......................................................... 56 open in new tab
13. Elektroforeza poliakrylamidowa białek w warunkach denaturujących SDS-PAGE 57 4.1. Przygotowanie żelu poliakrylamidowego ........................................................................ 57 4.2. Przygotowanie próbek do elektroforezy białkowej .................................................... 57 4.3. Barwienie i odbarwianie żeli poliakrylamidowych ..................................................... 57 open in new tab
14. Izolacja DNA z żelu agarozowego .................................................................................................. 57
15. Oczyszczanie DNA po reakcjach enzymatycznych ................................................................ 57
16. Amplifikacja genów kodujących fuzyjne polimerazy DNA ................................................ 58
17. Reakcje trawienia DNA enzymami restrykcyjnymi............................................................... 59
18. Reakcja Gibsona .................................................................................................................................... 59 10. Hodowle bakteryjne ......................................................................................................................... 60 10.1. Hodowle w pożywce płynnej............................................................................................... 60 10.2. Hodowle na podłożu stałym ................................................................................................ 60 open in new tab
19. Transformacja komórek E. coli rekombinantowym DNA ................................................ 60 11.1 Przygotowanie komórek kompetentnych E. coli ......................................................... 60 11.2 Transformacja komórek kompetentnych E. coli .......................................................... 61
20. Sprawdzenie obecności DNA insertu ........................................................................................ 61 12.1 Sprawdzenie obecności DNA insertu za pomocą analizy restrykcyjnej ............ 61 12.2 Sprawdzenie obecności DNA insertu za pomocą sekwencjonowania ............... 61
21. Ekspresja genów kodujących białka fuzyjne ......................................................................... 62 13.1 Optymalizacja warunków ekspresji .................................................................................. 62 13.2 Ekspresja genów kodujących fuzyjne polimerazy DNA w objętości 1000 ml pożywki .................................................................................................................................................... 62
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138. Walkusz R. Otrzymywanie, charakterystyka molekularna i zastosowanie dwudomenowego białka PriB Thermoanaerobacter tengcongensis. Praca magisterska. 2016; Politechnika Gdańska, Wydziała Chemiczny, Katedra biotechnologii Molekularnej i Mikrobiologii.
139. Śpibida M, Krawczyk B, Zalewska-Piątek B, Piątek R, Wysocka M, Olszewski M. Fusion of DNA-binding domain of Pyrococcus furiosus ligase with TaqStoffel DNA polymerase as a useful tool in PCR with difficult targets. Appl Microbiol Biotechnol. 2018;102(2):713-721. open in new tab
140. Olszewski M, Śpibida M, Bilek M, Krawczyk B. Fusion of Taq DNA polymerase with single-stranded DNA binding-like protein of Nanoarchaeum equitans- Expression and characterization. PLoS One. 2017;12(9):e0184162. open in new tab
141. Śpibida M, Krawczyk B, Olszewski M, Kur J. Modified DNA polymerases for PCR troubleshooting. J Appl Genet. 2017;58(1):133-142. open in new tab
142. Krawczyk B, Kur J, Stojowska-Swędrzyńska K, Śpibida M. Principles and applications of Ligation Mediated PCR methods for DNA-based typing of microbial organisms.Acta Biochim Pol. 2016;63(1):39-52. open in new tab
143. Nowak M, Olszewski M, Śpibida M, Kur J. Characterization of single-stranded DNA-binding proteins from the psychrophilic bacteria Desulfotalea psychrophila, Flavobacterium psychrophilum, Psychrobacter arcticus, Psychrobacter cryohalolentis, Psychromonas ingrahamii, Psychroflexus torquis, and Photobacterium profundum. BMC Microbiol. 2014 Apr 14;14:91 open in new tab
144. Śpibida M, Olszewski M, Krawczyk B, Zalewska-Piątek B., Piątek R."Fuzyjne polimerazy DNA jako użyteczne narzędzia w amplifikacji trudnych matryc", Konferencja Biomillenium, 6-9 IX 2017 open in new tab
145. Śpibida M, Krawczyk B, Olszewski M, Kur J, "Polimerazy fuzyjne jako nowoczesne narzędzia amplifikacji kwasów nukleinowych" XXVIII Zjazd Polskiego Towarzystwa Mikrobiologów. Mikrobiologia -nowe wyzwania, nowe możliwości, 25-27 IX 2016
146. Śpibida M, Krawczy B, Olszewski M, "Fuzyjna polimeraza DNA Taq o ulepszonych właściwościach użytecznych w diagnostyce molekularne", Ogólnopolska konferencja Mikrobiologia w Medycynie, Przemyśle i Ochronie Środowiska, Łódż, 24-25 X 2015 open in new tab
147. Postery 1. Walkusz R, Komarnicka K, Śpibida M, Olszewski M, "Otrzymywanie i charakterystyka molekularna oraz aplikacje dwudomenowego białka TtePrib" XXVIII Zjazd Polskiego Towarzystwa Mikrobiologów. Mikrobiologia -nowe wyzwania, nowe możliwości, 25-27 IX 2016
148. Śpibida M, Olszewski M, Krawczyk B "Fuzyjna polimeraza DNA Taq o zwiększonej oporności na inhibitory z próbek klinicznych", Biomed Session, 12.12 2015
149. Śpibida M, Krawczyk B, Olszewski M, "Taq DNA polymerase fused with DNA binding protein with increased resistance to inhibitors from clinical samples" Diagmol 2015 XVI Conference on Molecular biology in diagnostic of infectious diseases and biotechnology in Memory of Professor Władysław J.H. Kunicki- Goldfinger, 28.11.2015
150. Śpibida M, Olszewski M, Krawczy B, "Increased concentration of Taq DNA polymerase as a solution for GC-richtemplates from clinical and environmentalsamples", Acta Biochimica Polonica. Supplement, 6th International Weigl Conference on Microbiology, 8-10 VII 2015
151. Śpibida M, Walkusz R, Zalewski B, Maciejewska N, Olszewski M, Krawczyk B, "Taq DNA polymerases fused with single-stranded DNA binding protein", P.15.44, Acta Biochimica Polonica, suppl. 1, 295 -295, 2014, The BIO 2014
152. Congress Warsaw, Poland, September 9th -12th 2014 open in new tab
153. Śpibida M, Szemiako K, Krawczyk B , Olszewski M, Zalewska-Piątek B , Piątek R, "Otrzymywanie polimerazy Pfu DNA o zwiększonej oporności na inhibitory krwi użytecznej w diagnostyce molekularne", I Ogólnopolska Konferencja "Drobnoustroje w świecie człowieka -Drobnoustroje oportunistyczne" Bydgoszcz, 18-20 IX 2014 open in new tab
154. Szemiako K, Śpibida M, Krawczyk B, Olszewski M, "Zastosowanie metody T- RFLP do identyfikacji gatunkowej drożdżaków z rodzaju Candida", I Ogólnopolska Konferencja "Drobnoustroje w świecie człowieka - Drobnoustroje oportunistyczne" Bydgoszcz, 18-20 IX 2014 r open in new tab
155. Szemiako K, Śpibida M, Krawczyk B, Olszewski M, "Jednoczesna identyfikacja gatunkowa pięciu klinicznie istotnych drożdżaków z rodzaju Candida z wykorzystaniem reakcji multipleks PCR", I Ogólnopolska Konferencja "Drobnoustroje w świecie człowieka -Drobnoustroje oportunistyczne" Bydgoszcz, 18-20 IX 2014 open in new tab
156. Zalewska-Piątek B, Piątek R, Pilipczuk J, Śpibida M, Olszewski M, "Białko DRAD jako czynnik urowirulenty zlokalizowany na powierzchni komórek bakteryjnych szczepów Escherichia coli DR+", I Ogólnopolska Konferencja "Drobnoustroje w świecie człowieka -Drobnoustroje oportunistyczne" Bydgoszcz, 18-20 IX 2014
157. Śpibida M, Szemiako K, Krawczyk B, Olszewski M, Pilipczuk J, "Fuzja polimerazy DNA Taq z białkiem wiążącym DNA o zwiększonej użyteczności w diagnostyce molekularnej", I Ogólnopolska Konferencja "Drobnoustroje w świecie człowieka -Drobnoustroje oportunistyczne" Bydgoszcz, 18-20 IX 2014
158. Śpibida M, Krawczyk B, Olszewski M, "Taq DNA polymerase fused with single and double stranded DNA binding protein useful in molecular diagnostic and genetic engineering", p.173, 15th Konferencja "Molecular biology in diagnostics of infectious diseases and biotechnology" "DiagMol 2014" Warszawa, 25 X 2014
159. Walkusz R, Zalewski B, Śpibida M, Olszewski M, "Specific detection of Psilocybe cubensis DNA by PCR amplification of the gpd gene fragment", p. 175, 15th Konferencja "Molecular biology in diagnostics of infectious diseases and biotechnology" "DiagMol 2014" Warsaw, 25 X 2014
160. Zalewski B, Walkusz R, Śpibida M, Olszewski M, "PCR amplification of the conserved fragment of gpd gene used to specific detection of Psilocybe cubensis DNA", p. 185, 15th Konferencka "Molecular biology in diagnostics of infectious diseases and biotechnology" "DiagMol 2014" Warsaw, 25 X 2014
161. Śpibida M, Marszałkowska M, Olszewski M, Novel class of primosomal protein B from Clostridium thermocellum, P5.26, p. 54, 8 -11 X 2013, 5th Central European Congress of Life Sciences Eurobiotech 2013, Kraków
162. Marszałkowska M, Zakrzewska S, Śpibida M, Olszewski M, A novel DNA polymerase from hyperthermophilic bacterium Thermovibrio ammonificans: gene cloninig, expression, and characterization, P5.27, str. 55, 8 -11 X 2013, 5th Central European Congress of Life Sciences Eurobiotech 2013, Kraków open in new tab
163. Śpibida M, Marszałkowska M, Olszewski M, Novel primosomal protein B from Clostridium pasteurianum, P.3.35, str. 55, 2 -5 IX 2013,48th Congress of the Polish Biochemical Society, Toruń open in new tab
164. Olszewski M, Nowak M, Śpibida M, Characterization of single-stranded DNA- binding proteins from the psychrophilic bacteria, P3.24, str. 49, 2 -5 IX 2013, 48th Congress of the Polish Biochemical Society, Toruń
165. Olszewski M, Nowak M, Śpibida M, Kur J, Novel SSB protein from psychrophilic bacteria Pseudoalteromonas haloplanktis, 55th Polish Crystallographic Meeting, VI 27-29, 2013, Wrocław, B-2 open in new tab
166. Marszałowska M, Śpibida M, Olszewski M, cDiscovery of novel uvrA genes in Betaproteobacteriae, str. 59, 23 -25.11.2012: XIV National Academic Seminar of Biotechnology Students & IV International Students' Conference of Biotechnology, Gdańsk
167. Poleska M, Tarkowska A, Śpibida M, Olszewski M, Primosomal DNA replication proteins structure prediction using homology modeling, str. 117, 23 - 25.11.2012: XIV National Academic Seminar of Biotechnology Students & IV International Students' Conference of Biotechnology, Gdańsk
168. Żownir M, Bil M, Śpibida M, Kreft Ł, Olszewski M, "Discovery and identification of novel additional priB genes in bacteria", str. 116,23 -25.11.2012: XIV National Academic Seminar of Biotechnology Students & IV International Students' Conference of Biotechnology, Gdańsk
169. Śpibida M, Bil M, Żownir M, Kreft Ł,Olszewski M, "Novel single-stranded DNA binding proteins from Bacteria", P-30, str. 152, 08 -10.11.2012: VII International Conference of Young Naturalists "From Biotechnology to Environment Protection" -interdisciplinary meeting of young naturalists for students and PhD students, ZielonaGóra
170. Bil M, Kreft Ł, Śpibida M, Olszewski M, "Biodiversity of SSB proteins in the kingdom of Bacteria", P-31, str. 154, 08 -10.11.2012: VII International Conference of Young Naturalists "From Biotechnology to Environment Protection" -interdisciplinary meeting of young naturalists for students and PhD students, ZielonaGóra open in new tab

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