Abstract
Reakcja PCR już od wielu lat jest podstawowym narzędziem stosowanym w biotechnologii molekularnej. Nadal napotykamy jednak liczne problemy podczas jej przeprowadzania związane z trudnymi matrycami, obecnością inhibitorów itp. Optymalizacja reakcji PCR jest więc niezbędna w celu poprawy wyników. Jednym z rozwiązań tych problemów może być modyfikowanie polimeraz DNA. W powyższym artykule opisano problemy i rozwiązania zależne polimerazy DNA. Opisano również powody, które uzasadniają potrzebę modyfikacji polimeraz DNA, rodzaje modyfikacji, a także podjęto próbę określenia wpływu tych zmian na końcową efektywność polimeraz.
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- Category:
- Monographic publication
- Type:
- rozdział, artykuł w książce - dziele zbiorowym /podręczniku o zasięgu krajowym
- Title of issue:
- W : Enzymologia w obliczu wyzwań i możliwości XXI wieku strony 26 - 42
- Language:
- Polish
- Publication year:
- 2017
- Bibliographic description:
- Śpibida M., Olszewski M., Krawczyk B.: Modyfikacje polimeraz DNA jako rozwiązania problemów w reakcjach PCR// Enzymologia w obliczu wyzwań i możliwości XXI wieku/ ed. Beata A. Nowak, Monika Maciąg Lublin: Wydawnictwo Naukowe TYGIEL, 2017, s.26-42
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