High prevalence of Escherichia coli belonging to the B2+D phylogenetic group in inflammatory bowel disease

Roman Kotlowski; Charles Bernstein; Shadi Sepehri; Denis Krause

doi:10.1136/gut.2006.099796

High prevalence of Escherichia coli belonging to the B2+D phylogenetic group in inflammatory bowel disease

Abstract

BACKGROUND: It is not clear which species of bacteria may be involved in inflammatory bowel disease (IBD). One way of determining which bacteria might be likely candidates is to use culture-independent methods to identify microorganisms that are present in diseased tissues but not in controls. AIMS: (1) To assess the diversity of microbial communities of biopsy tissue using culture-independent methods; (2) to culture the bacteria found in the tissues of patients with IBD but not in the controls; (3) to identify potential virulence factors associated with cultured bacteria. METHODS: 84 biopsy specimens were collected from 15 controls, 13 patients with Crohn's disease (CD) and 19 patients with ulcerative colitis (UC) from a population-based case-control study. Ribosomal intergenic spacer analysis (RISA) was conducted to identify unique DNA bands in tissues from patients with CD and UC that did not appear in controls. RESULTS: RISA followed by DNA sequencing identified unique bands in biopsy specimens from patients with IBD that were classified as Escherichia coli. Targeted culture showed a significantly (p<0.05) higher number of Enterobacteriaceae in specimens from patients with IBD. The B2+D phylogenetic group, serine protease autotransporters (SPATE) and adherence factors were more likely to be associated with tissues from patients with UC and CD than with controls. CONCLUSIONS: The abundance of Enterobacteriaceae is 3-4 logs higher in tissues of patients with IBD and the B2+D phylogenetic groups are more prevalent in patients with UC and CD. The B2+D phylogenetic groups are associated with SPATE and adherence factors and may have a significant role in disease aetiology.

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Authors (4)

Roman Kotlowski (formerly: Roman Kotłowski) dr hab. inż.
Charles Bernstein Profesor
- University of Manitoba
Shadi Sepehri Dr
- University of Manitoba
Denis Krause Profesor
- University of Manitoba

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Category:: Articles
Type:: artykuł w czasopiśmie wyróżnionym w JCR
Published in:: GUT no. 56, pages 669 - 675,
ISSN: 0017-5749
Language:: English
Publication year:: 2007
Bibliographic description:: Kotłowski R., Bernstein C., Sepehri S., Krause D.: High prevalence of Escherichia coli belonging to the B2+D phylogenetic group in inflammatory bowel disease// GUT. -Vol. 56, nr. 5 (2007), s.669-675
DOI:: Digital Object Identifier (open in new tab) 10.1136/gut.2006.099796
Verified by:: Gdańsk University of Technology