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IN VITRO PROPAGATION OF RHODODENDRON TOMENTOSUM - AN ENDANGERED ESSENTIAL OIL BEARING PLANT FROM PEATLAND
Abstract
Rhododendron tomentosum Harmaja (formerly Ledum palustre L.) is a medicinal peat bog plant native to northern Europe, Asia and North America. This plant has a distinctive aroma thanks to the presence of essential oil, to which it also owes its anti-inflammatory, analgesic, antimicrobial and insecticidal properties. However, in Europe R. tomentosum is classified as an endangered species, mainly due to degradation of peatlands. In the present work, the micropropagation protocol for R. tomentosum was established for the first time, providing both an ex situ conservation tool and a means of continuous production of in vivo and in vitro plant material for further studies. R. tomentosum microshoots were initiated from leaf explants and further multiplied using Schenk-Hildebrandt (SH) medium supplemented with 9.84 μM 2iP and 1.00 μM TDZ. The shoots were elongated on the SH medium supplemented with 24.6 μM 2iP and subsequently rooted using the perlite substrate saturated with half-strength Woody Plant medium supplemented with 1.0% sucrose and 4.92 μM IBA. The regenerated plants were hardened on the phytohormone-free SH medium and acclimatized using 3:1:1 deacidified peat:perlite:gravel substrate. The identity of the mother plant was confirmed at morphological and molecular levels and Random Amplified Polymorphic DNA (RAPD) method was implemented to assess the genetic fidelity of the regenerants. The essential oil content of the maternal plant, in vitro shoots and the regenerants was determined by steam-distillation, and the obtained volatile fractions were analyzed by GC/MS.
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Authors (10)
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Anna Jesionek
- Medical University of Gdansk Department of Pharmacognosy
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Adam Kokotkiewicz
- Medical University of Gdansk Department of Pharmacognosy
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Paulina Włodarska
- Medical University of Gdansk Department of Pharmacognosy
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Natalia Filipowicz
- Medical University of Gdansk Department of Biology and Pharmaceutical Botany
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Adam Bogdan
- Medical University of Gdansk Department of Biology and Pharmaceutical Botany
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Renata Ochocka
- Medical University of Gdansk Department of Biology and Pharmaceutical Botany
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Adam Buciński
- Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun Department of Biopharmacy
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Maria Łuczkiewicz
- Medical University of Gdansk Department of Pharmacognosy
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- Accepted or Published Version
- DOI:
- Digital Object Identifier (open in new tab) 10.1515/abcsb-2016-0019
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Details
- Category:
- Articles
- Type:
- artykuł w czasopiśmie wyróżnionym w JCR
- Published in:
-
ACTA BIOLOGICA CRACOVIENSIA SERIES BOTANICA
no. 58,
pages 29 - 43,
ISSN: 0001-5296 - Language:
- English
- Publication year:
- 2016
- Bibliographic description:
- Jesionek A., Kokotkiewicz A., Włodarska P., Filipowicz N., Bogdan A., Ochocka R., Szreniawa-Sztajnert A., Zabiegała B., Buciński A., Łuczkiewicz M.: IN VITRO PROPAGATION OF RHODODENDRON TOMENTOSUM - AN ENDANGERED ESSENTIAL OIL BEARING PLANT FROM PEATLAND// ACTA BIOLOGICA CRACOVIENSIA SERIES BOTANICA. -Vol. 58, nr. 2 (2016), s.29-43
- DOI:
- Digital Object Identifier (open in new tab) 10.1515/abcsb-2016-0019
- Verified by:
- Gdańsk University of Technology
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