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Bioreactor shoot cultures of Rhododendron tomentosum (Ledum palustre) for a large-scale production of bioactive volatile compounds
Abstract
Rhododendron tomentosum Harmaja (Ledum palustre), a peat bog plant from Ericaceae family, has been used in traditional medicine as the anti-arthritis agent. Although modern researches confirm its anti-inflammatory properties, it remains threatened by habitat degradation and possibilities to collect this endangered species from its natural environment for further biological activity studies are limited. Therefore, R. tomentosum liquid in vitro cultures were established as the alternative source of that valuable plant material. Schenk–Hildebrandt medium with 24.60 μM 2-isopentenyladenine and 592.02 μM adenine provides intensive growth and proper morphology of the obtained microshoots. The R.tomentosum biomass was scaled up using the various bioreactors (immersion, temporary immersion and spraying systems) for better growth and improved volatile oil production. The largest biomass accumulation (fresh weight = 250 g l−1, growth index = 280, dry weight = 20 g l−1) and essential oil content (0.5% v/m) were achieved with application of commercially available RITA ® bioreactor. GC/MS analysis revealed the high content of p-cymene (6.9%), alloaromadendrene (5.5%), shyobunone (8.2%) and ledene oxide (II) (13.0%) in the volatile fractionobtained from RITA ® system. The biomass growth parameters and production profile in terms of essential oil and selected terpenoid compounds were determined during the 2 month period. The influence of culture conditions and bioreactor construction on the growth and volatile oil production in R. tomentosum biomasses was discussed.
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Authors (6)
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Anna Jesionek
- GUM
-
Adam Kokotkiewicz
- GUM
-
Paulina Włodarska
- GUM
-
Adam Buciński
- Nicolaus Copernicus University
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Maria Łuczkiewicz
- GUM
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Full text
- Publication version
- Accepted or Published Version
- DOI:
- Digital Object Identifier (open in new tab) 10.1007/s11240-017-1261-0
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Details
- Category:
- Articles
- Type:
- artykuł w czasopiśmie wyróżnionym w JCR
- Published in:
-
PLANT CELL TISSUE AND ORGAN CULTURE
no. 131,
edition 1,
pages 51 - 64,
ISSN: 0167-6857 - Language:
- English
- Publication year:
- 2017
- Bibliographic description:
- Jesionek A., Kokotkiewicz A., Włodarska P., Zabiegała B., Buciński A., Łuczkiewicz M.: Bioreactor shoot cultures of Rhododendron tomentosum (Ledum palustre) for a large-scale production of bioactive volatile compounds // PLANT CELL TISSUE AND ORGAN CULTURE. -Vol. 131, iss. 1 (2017), s.51-64
- DOI:
- Digital Object Identifier (open in new tab) 10.1007/s11240-017-1261-0
- Verified by:
- Gdańsk University of Technology
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