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Bioreactor shoot cultures of Rhododendron tomentosum (Ledum palustre) for a large-scale production of bioactive volatile compounds
Abstrakt
Rhododendron tomentosum Harmaja (Ledum palustre), a peat bog plant from Ericaceae family, has been used in traditional medicine as the anti-arthritis agent. Although modern researches confirm its anti-inflammatory properties, it remains threatened by habitat degradation and possibilities to collect this endangered species from its natural environment for further biological activity studies are limited. Therefore, R. tomentosum liquid in vitro cultures were established as the alternative source of that valuable plant material. Schenk–Hildebrandt medium with 24.60 μM 2-isopentenyladenine and 592.02 μM adenine provides intensive growth and proper morphology of the obtained microshoots. The R.tomentosum biomass was scaled up using the various bioreactors (immersion, temporary immersion and spraying systems) for better growth and improved volatile oil production. The largest biomass accumulation (fresh weight = 250 g l−1, growth index = 280, dry weight = 20 g l−1) and essential oil content (0.5% v/m) were achieved with application of commercially available RITA ® bioreactor. GC/MS analysis revealed the high content of p-cymene (6.9%), alloaromadendrene (5.5%), shyobunone (8.2%) and ledene oxide (II) (13.0%) in the volatile fractionobtained from RITA ® system. The biomass growth parameters and production profile in terms of essential oil and selected terpenoid compounds were determined during the 2 month period. The influence of culture conditions and bioreactor construction on the growth and volatile oil production in R. tomentosum biomasses was discussed.
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Autorzy (6)
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Anna Jesionek
- GUM
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Adam Kokotkiewicz
- GUM
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Paulina Włodarska
- GUM
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Bożena Zabiegała prof. dr hab. inż.
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Adam Buciński
- Nicolaus Copernicus University
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Maria Łuczkiewicz
- GUM
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Pełna treść
- Wersja publikacji
- Accepted albo Published Version
- DOI:
- Cyfrowy identyfikator dokumentu elektronicznego (otwiera się w nowej karcie) 10.1007/s11240-017-1261-0
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Informacje szczegółowe
- Kategoria:
- Publikacja w czasopiśmie
- Typ:
- artykuł w czasopiśmie wyróżnionym w JCR
- Opublikowano w:
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PLANT CELL TISSUE AND ORGAN CULTURE
nr 131,
wydanie 1,
strony 51 - 64,
ISSN: 0167-6857 - Język:
- angielski
- Rok wydania:
- 2017
- Opis bibliograficzny:
- Jesionek A., Kokotkiewicz A., Włodarska P., Zabiegała B., Buciński A., Łuczkiewicz M.: Bioreactor shoot cultures of Rhododendron tomentosum (Ledum palustre) for a large-scale production of bioactive volatile compounds // PLANT CELL TISSUE AND ORGAN CULTURE. -Vol. 131, iss. 1 (2017), s.51-64
- DOI:
- Cyfrowy identyfikator dokumentu elektronicznego (otwiera się w nowej karcie) 10.1007/s11240-017-1261-0
- Weryfikacja:
- Politechnika Gdańska
wyświetlono 161 razy
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