Evaluation of the yield, chemical composition and biological properties of essential oil from bioreactor-grown cultures of Salvia apiana microshoots - Publikacja - MOST Wiedzy

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Evaluation of the yield, chemical composition and biological properties of essential oil from bioreactor-grown cultures of Salvia apiana microshoots

Abstrakt

Microshoot cultures of the North American endemic Salvia apiana were established for the first time and evaluated for essential oil production. Stationary cultures, grown on Schenk-Hildebrandt (SH) medium, supplemented with 0.22 mg/L thidiazuron (TDZ), 2.0 mg/L 6-benzylaminopurine and 3.0% (w/v) sucrose, accumulated 1.27% (v/m dry weight) essential oil, consisting mostly of 1,8-cineole, β-pinene, α-pinene, β-myrcene and camphor. The microshoots were adapted to agitated culture, showing biomass yields up to ca. 19 g/L. Scale-up studies demonstrated that S. spiana microshoots grow well in temporary immersion systems (TIS). In the RITA bioreactor, up to 19.27 g/L dry biomass was obtained, containing 1.1% oil with up to ca. 42% cineole content. The other systems employed, i.e. Plantform (TIS) and a custom made spray bioreactor (SGB), yielded ca. 18 and 19 g/L dry weight, respectively. The essential oil content of Plantform and SGB-grown microshoots was comparable to RITA bioreactor, however, the content of cineole was substantially higher (ca. 55%). Oil samples isolated from in vitro material proved to be active in acetylcholinesterase (up to 60.0% inhibition recorded for Plantform-grown microshoots), as well as hyaluronidase and tyrosinase-inhibitory assays (up to 45.8 and 64.5% inhibition observed in the case of the SGB culture).

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Autorzy (10)

  • Zdjęcie użytkownika  Agata Krol

    Agata Krol

    • Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Gdansk
  • Zdjęcie użytkownika  Adam Kokotkiewicz

    Adam Kokotkiewicz

    • Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Gdansk
  • Zdjęcie użytkownika  Marcin Gorniak

    Marcin Gorniak

    • Department of Evolutionary Genetics and Biosystematics, Faculty of Biology, University of Gdansk
  • Zdjęcie użytkownika  Aleksandra M. Naczk

    Aleksandra M. Naczk

    • Department of Evolutionary Genetics and Biosystematics, Faculty of Biology, University of Gdansk
  • Zdjęcie użytkownika  Jakub Gebalski

    Jakub Gebalski

    • Department of Pharmaceutical Botany and Pharmacognosy, Faculty of Pharmacy, Ludwik Rydygier Collegium Medicum, Nicolaus Copernicus University
  • Zdjęcie użytkownika  Filip Graczyk

    Filip Graczyk

    • Department of Pharmaceutical Botany and Pharmacognosy, Faculty of Pharmacy, Ludwik Rydygier Collegium Medicum, Nicolaus Copernicus University
  • Zdjęcie użytkownika  Daniel Zaluski

    Daniel Zaluski

    • Department of Pharmaceutical Botany and Pharmacognosy, Faculty of Pharmacy, Ludwik Rydygier Collegium Medicum, Nicolaus Copernicus University
  • Zdjęcie użytkownika  Adam Bucinski

    Adam Bucinski

    • Department of Biopharmacy, Faculty of Pharmacy, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun
  • Zdjęcie użytkownika  Maria Luczkiewicz

    Maria Luczkiewicz

    • Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Gdansk

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Informacje szczegółowe

Kategoria:
Publikacja w czasopiśmie
Typ:
artykuły w czasopismach
Opublikowano w:
Scientific Reports nr 13,
ISSN: 2045-2322
Język:
angielski
Rok wydania:
2023
Opis bibliograficzny:
Krol A., Kokotkiewicz A., Gorniak M., Naczk A. M., Zabiegała B., Gebalski J., Graczyk F., Zaluski D., Bucinski A., Luczkiewicz M.: Evaluation of the yield, chemical composition and biological properties of essential oil from bioreactor-grown cultures of Salvia apiana microshoots// Scientific Reports -,iss. 13/7141 (2023),
DOI:
Cyfrowy identyfikator dokumentu elektronicznego (otwiera się w nowej karcie) 10.1038/s41598-023-33950-1
Źródła finansowania:
  • Publikacja bezkosztowa
Weryfikacja:
Politechnika Gdańska

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