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Isolation of Human Progenitor Epidermal Cells on Collagen Type IV and Analysis of Their Markers with Flow Cytometry and PCR Methods
Abstract
Epidermal stem cells, located in the skin, together with keratinocytes are transplanted in regenerative therapies, e.g., for the treatment of burns or other wounds. Here, we describe the protocol of their enzymatic isolation from human skin. It includes separation of the epidermis form the dermis by incubation with dispase followed by cell isolation for epidermis by digestion with trypsin. Cell isolated with this method can be seeded on collagen IV-coated dishes. The methods of analysis of epidermal stem cells markers (e.g., CD71, CD29) with flow cytometry and RT-PCR are also included.
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Authors (4)
-
Milena Deptula
- Gdański Uniwersytet Medyczny
-
Małgorzata Zawrzykraj mgr
- Gdański Uniwersytet Medyczny
-
Michał Pikuła Prof. dr hab.
- Gdański Uniwersytet Medyczny
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Keywords
Details
- Category:
- Monographic publication
- Type:
- rozdział, artykuł w książce - dziele zbiorowym /podręczniku w języku o zasięgu międzynarodowym
- Language:
- English
- Publication year:
- 2024
- Bibliographic description:
- Deptula M., Zawrzykraj M., Słonimska P., Pikuła M.: Isolation of Human Progenitor Epidermal Cells on Collagen Type IV and Analysis of Their Markers with Flow Cytometry and PCR Methods// / Nowy Jork, Stany Zjednoczone: SpringerLink, 2024,
- DOI:
- Digital Object Identifier (open in new tab) 10.1007/7651_2024_541
- Sources of funding:
-
- COST_FREE
- Verified by:
- Gdańsk University of Technology
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