Rapid Assays for Specific Detection of Fungi of Scopulariopsis and Microascus Genera and Scopulariopsis brevicaulis Species - Publication - Bridge of Knowledge

Your account

login

Search

Rapid Assays for Specific Detection of Fungi of Scopulariopsis and Microascus Genera and Scopulariopsis brevicaulis Species

Abstract

Purpose Fungi of Scopulariopsis and Microascus genera cause a wide range of infections, with S. brevicaulis being the most prevalent aetiological agent of mould onychomycosis. Proper identification of these pathogens requires sporulating culture, which considerably delays the diagnosis. So far, sequencing of rDNA regions of clinical isolates has produced ambiguous results due to the lack of reference sequences in publicly available databases. Thus, there is a clear need for the development of new molecular methods that would provide simple, rapid and highly specific identification of Scopulariopsis and Microascus species. The objective of this study was to develop simple and fast assays based on PCR and real-time PCR for specific detection of fungi from Scopulariopsis and Microascus genera, and separately, S. brevicaulis species.

Citations

  • 8

    CrossRef

  • 0

    Web of Science

  • 7

    Scopus

Authors (3)

Cite as

Full text

download paper
downloaded 20 times
Publication version
Accepted or Published Version
DOI:
Digital Object Identifier (open in new tab) 10.1007/s11046-016-0008-5
License
Creative Commons: CC-BY open in new tab

Keywords

Details

Category:
Articles
Type:
artykuł w czasopiśmie wyróżnionym w JCR
Published in:
MYCOPATHOLOGIA pages 465 - 474,
ISSN: 0301-486X
Language:
English
Publication year:
2016
Bibliographic description:
Kordalewska M., Jagielski T., Brillowska-Dąbrowska A.: Rapid Assays for Specific Detection of Fungi of Scopulariopsis and Microascus Genera and Scopulariopsis brevicaulis Species// MYCOPATHOLOGIA. -, nr. 181 (2016), s.465-474
DOI:
Digital Object Identifier (open in new tab) 10.1007/s11046-016-0008-5
Verified by:
Gdańsk University of Technology

seen 147 times

Recommended for you

PCR-RFLP assays for species-specific identification of fungi belonging to Scopulariopsis and related genera

  • M. Kordalewska,
  • J. Kalita,
  • Z. Bakuła
  • + 2 authors
2019

A New Double Digestion Ligation Mediated Suppression PCR Method for Simultaneous Bacteria DNA-Typing and Confirmation of Species: An Acinetobacter sp. Model

2014

Typing of Scopulariopsis and Microascus fungi by Random Amplified Polymorphic DNA (RAPD)

2015

PCR and real-time PCR approaches to the identification of Arthroderma otae species Microsporum canis and Microsporum audouinii/Microsporum ferrugineum

2016
Meta Tags