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• Immobilization on magnetic nanoparticles of therecombinant trehalose synthase from Deinococcusgeothermalis

  Publication

  • A. Panek
  • O. Pietrow-Tobiszewska
  • J. Synowiecki
  • P. Filipkowski

  - FOOD AND BIOPRODUCTS PROCESSING - Year 2013

  tIn our study the gene encoding trehalose synthase from Deinococcus geothermalis was cloned and overexpressed inEscherichia coli Rosetta (DE3)pLysS. Wild-type trehalose synthase has been purified from host protein after cell dis-ruption and precipitation at 20% ammonium sulphate saturation. Recombinant trehalose synthase was immobilizedonto glutaraldehyde activated silanized magnetic ferrous-ferric oxide by using covalent binding...

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