Year 2014

• Phenotypic consequences of LYS4 gene disruption in Candida albicans

  Publication

  • I. Gabriel
  • K. Kur
  • S. S. Laforce-Nesbitt
  • A. S. Pulickal
  • J. M. Bliss
  • S. Milewski

  - YEAST - Year 2014

  A BLAST search of the Candida Genome Database with the Saccharomyces cerevisiae LYS4 sequence known to encode homoaconitase (HA) revealed ORFs 19.3846 and 19.11327. Both alleles of the LYS4 gene were sequentially disrupted in Candida albicans BWP17 cells using PCR-based methodology. The null lys4Δ mutant exhibited lysine auxotrophy in minimal medium but was able to grow in the presence of L-Lys and α-aminoadipate, an intermediate...

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