Cytotoxicity of chitosan hydrogels aginst L929 cells - Open Research Data - Bridge of Knowledge

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Cytotoxicity of chitosan hydrogels aginst L929 cells

Description

This study presents absorbance values of formazan product (converted from MTT) which corresponds to the cytotoxicity of chitosan compounds (dissolved in carbonic acid, dissolved in carbonic acid endotoxin free, and dissolved in hydroxyacetic acid)  aginst adult mouse fibroblast L929 cells. The experiments were carried out according to the ISO 10993-5:2009(E) standard.

Cytotoxicity of compounds was measured in three different dilution factors: 1:1; 1:3 and 1:6. Briefly, L929 cells were seeded at 104 per well. After 24 h of incubation, the medium was replaced with new medium containing compounds and incubated for 24 h. Following 24 h of the treatment, the medium was changed to fresh containing 1 mg/mL MTT and incubated for 2 h. Next, the medium was removed and formazan crystals were dissolved in 100 µL of isopropanol. The absorbance was measured using a spectrophotometer (540 nm). Based on the results % of viability was determined, assuming that the control was 100%.

Dataset file

Cytotoxicity of CH.CO2, CH.KW and CH.OCZ against L929 cells in different dilution factors.xlsx
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License:
Creative Commons: by 4.0 open in new tab
CC BY
Attribution
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Details

Year of publication:
2021
Verification date:
2021-10-29
Dataset language:
English
Fields of science:
  • Chemical sciences (Natural sciences)
  • Chemical engineering (Engineering and Technology)
  • Biomedical engineering (Engineering and Technology)
DOI:
DOI ID 10.34808/hjkc-2318 open in new tab
Verified by:
Gdańsk University of Technology

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