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Bioactive hybrid metal-organic framework (MOF)-based nanosensors for optical detection of recombinant SARS-CoV-2 spike antigen
Abstract
Fast, efficient, and accurate detection of SARS-CoV-2 spike antigen is pivotal to control the spread and reduce themortality of COVID-19. Nevertheless, the sensitivity of available nanobiosensors to detect recombinant SARS-CoV-2 spike antigen seems insufficient. As a proof-of-concept, MOF-5/CoNi2S4 is developed as a low-cost, safe, and bioactive hybrid nanostructure via the one-pot high-gravity protocol. Then, the porphyrin, H2TMP, was attached to the surface of the synthesized nanomaterial to increase the porosity for efficient detection of recombinant SARS-CoV-2 spike antigen. AFMresults approved roughness in different ranges, including 0.54 to 0.74 μm and 0.78 to≈0.80 μm, showing good physical interactions with the recombinant SARS-CoV-2 spike antigen. MTT assay was performed and compared to the conventional synthesis methods, including hydrothermal, solvothermal, and microwave-assisted methods. The synthesized nanodevices demonstrated above 88%relative cell viability after 24 h and even 48 h of treatment. Besides, the ability of the synthesized nanomaterials to detect the recombinant SARS-CoV-2 spike antigen was investigated, with a detection limit of 5 nM. The in-situ synthesized nanoplatforms exhibited low cytotoxicity, high biocompatibility, and appropriate tunability. The fabricated nanosystems seempromising for future surveys as potential platforms to be integrated into biosensors.
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Authors (14)
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Navid Rabiee
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Yousef Fatahi
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Sepideh Ahmadi
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Nikzad Aabbariki
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Amirhossein Ojaghi
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Mohammad Rabiee
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Fatemeh Radmanesh
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Rassoul Dinarvand
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Mojtaba Bagherzadeh
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Ebrahim Mostafavi
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Milad Ashrafizadeh
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Pooyan Makvandi
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Eder C. Lima
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Details
- Category:
- Articles
- Type:
- artykuły w czasopismach
- Published in:
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SCIENCE OF THE TOTAL ENVIRONMENT
no. 825,
ISSN: 0048-9697 - Language:
- English
- Publication year:
- 2022
- Bibliographic description:
- Rabiee N., Fatahi Y., Ahmadi S., Aabbariki N., Ojaghi A., Rabiee M., Radmanesh F., Dinarvand R., Bagherzadeh M., Mostafavi E., Ashrafizadeh M., Makvandi P., Lima E. C., Saeb M.: Bioactive hybrid metal-organic framework (MOF)-based nanosensors for optical detection of recombinant SARS-CoV-2 spike antigen// SCIENCE OF THE TOTAL ENVIRONMENT -Vol. 825, (2022), s.153902-
- DOI:
- Digital Object Identifier (open in new tab) 10.1016/j.scitotenv.2022.153902
- Sources of funding:
-
- COST_FREE
- Verified by:
- Gdańsk University of Technology
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