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Cloning, expression, purification and characterization of recombinant trehalose synthase from Deinococcus radiodurans.
Abstract
Trehalose (α-D-glucopyranosyl-1,1-α-D-glucopyranoside) is a nonreducing disacharide in which the two glucose molecules are linked trough a α-1,1-glycosidic bond. Trehalose is readily hydrolyzed to glucose and can be used as a reserve of that sugar in the cell. The presence of trehalose was found in the cells of fungi and yeasts, bacteria, nematodes, insects, eggs, pupae and some plants. The characteristics of trehalose make it a potential source of many applications in medical, veterinary, phahrmaceutical and food industry.Biosynthesis of trehalose is carried out various known methabolic pathways, including the use of trehalose synthase. Bacteria of the genus Deinococcus are microorganisms which produce trehalose synthase (DraST). The aim of this study was isolation and cloning of trehalose synthase gene derived from D.radiodurans to the expressive vectors in the system of Tabor-Studier and arabinose system and its expression in different hosts.
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- Category:
- Articles
- Type:
- artykuł w czasopiśmie indeksowanym TR Master Journal List
- Published in:
-
Acta Biochimica Polonica
no. 57,
ISSN: 0001-527X - Language:
- English
- Publication year:
- 2010
- Bibliographic description:
- Filipkowski P., Licznerska K., Panek A., Pietrow O., Synowiecki J.: Cloning, expression, purification and characterization of recombinant trehalose synthase from Deinococcus radiodurans.// Acta Biochimica Polonica. -Vol. 57., nr. suppl. 3 (2010),
- Verified by:
- Gdańsk University of Technology
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