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Epitope Mapping of BmpA and BBK32 Borrelia burgdorferi Sensu Stricto Antigens for the Design of Chimeric Proteins with Potential Diagnostic Value
Abstract
Lyme disease is a tick-borne zoonosis caused by Gramnegative bacteria belonging to the Borrelia burgdorferi sensu lato (s.l.) group. In this study, IgM- and IgG-specific linear epitopes of two B. burgdorferi sensu stricto (s.s.) antigens BmpA and BBK32 were mapped using a polypeptide array. Subsequently, two chimeric proteins BmpABBK32-M and BmpA-BBK32-G were designed to validate the construction of chimeras using the identified epitopes for the detection of IgM and IgG, respectively, by ELISA. IgG-ELISA based on the BmpABBK32-G antigen showed 71% sensitivity and 95% specificity, whereas a slightly lower diagnostic utility was obtained for IgM-ELISA based on BmpA-BBK32-M, where the sensitivity was also 71% but the specificity decreased to 89%. The reactivity of chimeric proteins with nondedicated antibodies was much lower. These results suggest that the identified epitopes may be useful in the design of new forms of antigens to increase the effectiveness of Lyme disease serodiagnosis. It has also been proven that appropriate selection of epitopes enables the construction of chimeric proteins exhibiting reactivity with a specific antibody isotype.
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Authors (6)
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Beata Fiecek mgr
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Marinela Contreras Rojo Doktor
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Full text
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- Accepted or Published Version
- DOI:
- Digital Object Identifier (open in new tab) 10.1021/acsinfecdis.3c00258
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- Category:
- Articles
- Type:
- artykuły w czasopismach
- Published in:
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ACS Infectious Diseases
no. 9,
pages 2160 - 2172,
ISSN: 2373-8227 - Language:
- English
- Publication year:
- 2023
- Bibliographic description:
- Grąźlewska W., Holec-Gąsior L., Sołowińska K., Chmielewski T., Fiecek B., Contreras Rojo M.: Epitope Mapping of BmpA and BBK32 Borrelia burgdorferi Sensu Stricto Antigens for the Design of Chimeric Proteins with Potential Diagnostic Value// ACS Infectious Diseases -,iss. 9(10) (2023), s.2160-2172
- DOI:
- Digital Object Identifier (open in new tab) 10.1021/acsinfecdis.3c00258
- Sources of funding:
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- COST_FREE
- Verified by:
- Gdańsk University of Technology
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