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Genetic and biochemical characterization of yeasts isolated from Antarctic soil samples

Abstract

The Polish Arctowski Station is situated in the maritime Antarctic on the western shore ot' Admiralty Bay and encompasses terrestrial habitats which are not perma-nently covered by ice, in contrast to morę than 90% of the island's surface area. Over the past several decades, stud-ies exploring the soils of those habitats have revealed a considerable diversity of bacteria, filamentous fungi, and, to a lesser extent, yeasts; however, characterization of this complex microbiome, especially at the rnolecular level, is still far from satisfactory. The isolates were assigned to their respective genera and species based on genetic analy-sis of the D1/D2 and ITS1-5.8S-ITS2 regions of rDNA. In the studied soil samples, the most abundant microorgan-isms belonged to the genera Cryptococcus, Rhodotorula, and Debaryomyces. Physiological and biochemical analy-sis of Cryptococcus gilvescens (pro tempore Gojfeauzyma gihescens) and Rhodotorula mucilaginosa showed only a limited level of intraspecies diversity. Cellular DNA con-tent and karyotypes were determined using flow cytometry and pulsed-field gel electrophoresis for several selected strains. For the first time, genome size and electrophoretic karyotypes were investigated in C. gihescens (pro tem G. gihescens), Cryptococcus saitoi (pro tem Naganiskia globosa), Cryptococcus gastricus (pro tem Gojfeauzyma gastrica), and Cryptococcus albidus (pro tem Naganishia albida). In addition, plate tests showed Antarctic yeasts to be a potential source of biotechnologically important en/.ymes. This study in biodiversity, presenting physiologi¬cal and rnolecular characterization of psychrotolerant yeast strains isolated from the soils of western Admiralty Bay, contributes to a better understanding of the microbial ecol-ogy of this uniąue ecosystem.

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Authors (8)

  • Photo of  Aneta Białkowska

    Aneta Białkowska

    • Politechnika Łódzka,Wydział Biotechnologii i Nauk o Żywności Instytut Biochemii Technicznej
  • Photo of  Katarzyna Szulczewska

    Katarzyna Szulczewska

    • Politechnika Łódzka,Wydział Biotechnologii i Nauk o Żywności Instytut Biochemii Technicznej
  • Photo of  Joanna Krysiak

    Joanna Krysiak

    • Politechnika Łódzka,Wydział Biotechnologii i Nauk o Żywności Instytut Biochemii Technicznej
  • Photo of dr inż. Tomasz Florczak

    Tomasz Florczak dr inż.

    • Politechnika Łódzka,Wydział Biotechnologii i Nauk o Żywności
  • Photo of mgr inż. Ewa Gromek

    Ewa Gromek mgr inż.

    • Politechnika Łódzka,Wydział Biotechnologii i Nauk o Żywności Instytut Biochemii Technicznej
  • Photo of  Hassan Kassassir

    Hassan Kassassir

    • Uniwersytet Medyczny w Łodzi, Zakład Zaburzeń Krzepnięcia Krwi Katedra Nauk Biomedycznych
  • Photo of prof. dr hab. Marianna Turkiewicz

    Marianna Turkiewicz prof. dr hab.

    • Politechnika Łódzka,Wydział Biotechnologii i Nauk o Żywności Instytut Biochemii Technicznej

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Category:
Articles
Type:
artykuł w czasopiśmie wyróżnionym w JCR
Published in:
POLAR BIOLOGY no. 38, pages 1 - 17,
ISSN: 0722-4060
Language:
English
Publication year:
2017
Bibliographic description:
Białkowska A., Szulczewska K., Krysiak J., Florczak T., Gromek E., Kassassir H., Kur J., Turkiewicz M.: Genetic and biochemical characterization of yeasts isolated from Antarctic soil samples// POLAR BIOLOGY. -Vol. 38, (2017), s.1-17
DOI:
Digital Object Identifier (open in new tab) 10.1007/s00300-017-2102-7
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