Improved cytotoxicity and preserved level of cell death induced in colon cancer cells by doxorubicin after its conjugation with iron-oxide magnetic nanoparticles

Ewa Augustin; Bartłomiej Czubek; Anna Maria Nowicka; Agata Kowalczyk; Zbigniew Stojek; Zofia Mazerska

doi:10.1016/j.tiv.2016.02.009

Improved cytotoxicity and preserved level of cell death induced in colon cancer cells by doxorubicin after its conjugation with iron-oxide magnetic nanoparticles

Abstract

A promising strategy for overcoming the problem of limited efficacy in antitumor drug delivery and in drug release is the use of a nanoparticle-conjugated drug. Doxorubicin (Dox) anticancer chemotherapeutics has been widely studied in this respect, because of severe cardiotoxic side effects. Here, we investigated the cytotoxic effects, the uptake process, the changes in cell cycle progression and the cell death processes in the presence of iron-oxide magnetic nanoparticles and doxorubicin conjugates (Dox-Nps) in human colon HT29 cells. The amount of Dox participated in biological action of Dox-Nps was determined by cyclic voltammetry and thermogravimetric measurements. The cytotoxicity of Dox-Nps was shown to be two/three times higher than free Dox, whereas Nps alone did not inhibit cell proliferation. Dox-Nps were observed to penetrate cancer cells with higher efficacy than free Dox, what could be a consequence of Dox-Nps aggregation with proteins in culture medium and/or with cell surface. The treatment of HT29 cells with Dox-Nps and Dox at IC50 concentration resulted in G2/M arrest followed by late apoptosis and necrosis. Summing up, the application of iron-oxide magnetic nanoparticles improved Dox-Nps cell penetration compared to free Dox and achieved the cellular response to Dox-Nps conjugates similar to that of Dox alone.

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Authors (6)

Ewa Augustin dr hab.
Bartłomiej Czubek
Anna Maria Nowicka
- Uniwersytet Warszawski, Wydział Chemii Laboratorium teorii i zastosowania elektrod
Agata Kowalczyk
- Uniwersytert Warszawski, Wydział Chemii Laboratorium teorii i zastosowania elektrod
Zbigniew Stojek
- Uniwersytet warszawski, Wydział Chemii Laboratorium teorii i zastosowania elektrod
Zofia Mazerska prof. dr hab. inż.

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Category:: Articles
Type:: artykuł w czasopiśmie wyróżnionym w JCR
Published in:: TOXICOLOGY IN VITRO no. 33, pages 45 - 53,
ISSN: 0887-2333
Language:: English
Publication year:: 2016
Bibliographic description:: Augustin E., Czubek B., Nowicka a., Kowalczyk A., Stojek Z., Mazerska Z.: Improved cytotoxicity and preserved level of cell death induced in colon cancer cells by doxorubicin after its conjugation with iron-oxide magnetic nanoparticles// TOXICOLOGY IN VITRO. -Vol. 33, (2016), s.45-53
DOI:: Digital Object Identifier (open in new tab) 10.1016/j.tiv.2016.02.009
Verified by:: Gdańsk University of Technology