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Incorporation of doxorubicin into plant-derived nanovesicles: process monitoring and activity assessment
Abstract
Extracellular vesicles (EVs) are an experimental class of drug carriers. Alternative sources of EVs are currently being explored to overcome limitations related to their manufacturing from mesenchymal stem cells. In this work, Citrus limon-derived EVs were tested as carriers for the widely used chemotherapeutic drug – doxorubicin (DOX). Capillary electrophoresis (CE) and nanoplasmonic sensing (NPS) were developed for the quality control of DOX–EV preparations. It was found that the CE method enables simultaneous detection of free and incorporated DOX and allows assessing the stability of the preparations and the drug leakage. NPS, on the other hand, demonstrated that DOX is accumulated in the interfacial region of the carrier. The activity of DOX-loaded EVs was tested on HeLa (cervical cancer cells) and HEK293T (human embryonic kidney cells) cell lines. It was found that DOX incorporation into plant-derived EVs virtually does not affect the drug’s cytotoxicity to HeLa cells but significantly decreases DOX activity against HEK293T cell line.
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Authors (14)
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Aleksandra Steć
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Monika Targońska
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Shishir Jaikishan
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Rui Chen
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Piotr Mucha dr hab.
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Grzegorz S. Czyrski
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Jacek Jasiecki
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Agata Płoska
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Andrea Heinz
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Susanne K. Wiedmer
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Krzysztof Waleron
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Bartosz Wielgomas
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Szymon Dziomba
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Full text
- Publication version
- Accepted or Published Version
- DOI:
- Digital Object Identifier (open in new tab) 10.1080/10717544.2024.2439272
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Details
- Category:
- Articles
- Type:
- artykuły w czasopismach
- Published in:
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DRUG DELIVERY
no. 32,
pages 1 - 11,
ISSN: 1071-7544 - Language:
- English
- Publication year:
- 2025
- Bibliographic description:
- Steć A., Targońska M., Jaikishan S., Chen R., Mucha P., Czyrski G. S., Jasiecki J., Płoska A., Heinz A., Wiedmer S. K., Kalinowski L., Waleron K., Wielgomas B., Dziomba S.: Incorporation of doxorubicin into plant-derived nanovesicles: process monitoring and activity assessment// DRUG DELIVERY -Vol. 32,iss. 1 (2025),
- DOI:
- Digital Object Identifier (open in new tab) 10.1080/10717544.2024.2439272
- Verified by:
- Gdańsk University of Technology
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