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Abstract
Despite the abundance of available cell lines, nearly 70% of all recombinant therapeutic proteins today are produced in Chi-nese hamster ovary (CHO) cells. The impact of protein overproduction on the secretion of exosomes by CHO cells has been investigated here. Increased secretion of extracellular vesicles (EVs) by protein overexpressing CHO cells was demonstrated with protein content assay, nanoparticle tracking analysis, and capillary electrophoresis. Our results revealed that a protein overproduction might induce EVs secretion, which might be accompanied by the sequestration and loading of overexpressed proteins into the exosomes. These findings are of vital importance for the manufacturing of therapeutics in CHO expres-sion systems due to the risk of product loss during downstream processing of culture medium as well as the application of exosomes as nanocarriers of therapeutic proteins. The study indicates also the importance of culturing process control.
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Authors (10)
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Aleksandra Steć
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Monika Targońska
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Edyta Karkosińska
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Monika Słowik
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Agata Płoska
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Bartosz Wielgomas
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Krzysztof Waleron
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Jacek Jasiecki
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Szymon Dziomba
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Full text
- Publication version
- Accepted or Published Version
- DOI:
- Digital Object Identifier (open in new tab) 10.1007/s00216-023-04725-4
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Details
- Category:
- Articles
- Type:
- artykuły w czasopismach
- Published in:
-
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
no. 415,
pages 3167 - 3176,
ISSN: 1618-2642 - Language:
- English
- Publication year:
- 2023
- Bibliographic description:
- Steć A., Targońska M., Karkosińska E., Słowik M., Płoska A., Kalinowski L., Wielgomas B., Waleron K., Jasiecki J., Dziomba S.: Protein overproduction alters exosome secretion in Chinese hamster ovary cells// ANALYTICAL AND BIOANALYTICAL CHEMISTRY -Vol. 415,iss. 16 (2023), s.3167-3176
- DOI:
- Digital Object Identifier (open in new tab) 10.1007/s00216-023-04725-4
- Sources of funding:
-
- This research was funded by the Polish Ministry of Educa-tion and Science, grants no. 664/514/61/71-1204 and 10/E-389/SPUB/SP/2020.
- Verified by:
- Gdańsk University of Technology
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