Metabolic activity of tree saps of different origin towards cultured human cells in the light of grade correspondence analysis and multiple regression modeling

Artur Wnorowski; Maciej Bilek; Kinga Stawarczyk; Michał Gostkowski; Marcin Olszewski; Magdalena Wójciak-Kosior; Ireneusz Sowa

doi:10.5586/asbp.3545

Metabolic activity of tree saps of different origin towards cultured human cells in the light of grade correspondence analysis and multiple regression modeling

Abstract

Tree saps are nourishing biological media commonly used for beverage and syrup production. Although the nutritional aspect of tree saps is widely acknowledged, the exact relationship between the sap composition, origin, and effect on the metabolic rate of human cells is still elusive. Thus, we collected saps from seven different tree species and conducted composition-activity analysis. Saps from trees of Betulaceae, but not from Salicaceae, Sapindaceae, nor Juglandaceae families, were increasing the metabolic rate of HepG2 cells, as measured using tetrazolium-based assay. Content of glucose, fructose, sucrose, chlorides, nitrates, sulphates, fumarates, malates, and succinates in sap samples varied across different tree species. Grade correspondence analysis clustered trees based on the saps’ chemical footprint indicating its usability in chemotaxonomy. Multiple regression modeling showed that glucose and fumarate present in saps from silver birch (Betula pendula Roth.), black alder (Alnus glutinosa Gaertn.), and European hornbeam (Carpinus betulus L.) are positively affecting the metabolic activity of HepG2 cells.

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Authors (7)

Artur Wnorowski dr
- Medical University of Lublin Department of Biopharmacy, Faculty of Pharmacy
Maciej Bilek
- University of Rzeszów Department of Food and Agriculture Production Engineering, Faculty of Biology and Agriculture
Kinga Stawarczyk
- University of Rzeszów Department of Botany, Institute of Applied Biotechnology and Basic Sciences
Michał Gostkowski
- Warsaw University of Life Sciences – SGGW Faculty of Applied Informatics and Mathematics
Marcin Olszewski dr hab. inż.
Magdalena Wójciak-Kosior
- Medical University of Lublin Department of Analytical Chemistry, Faculty of Pharmacy
Ireneusz Sowa
- Medical University of Lublin Department of Analytical Chemistry, Faculty of Pharmacy

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DOI:: Digital Object Identifier (open in new tab) 10.5586/asbp.3545
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Category:: Articles
Type:: artykuł w czasopiśmie wyróżnionym w JCR
Published in:: ACTA SOCIETATIS BOTANICORUM POLONIAE no. 86, pages 1 - 10,
ISSN: 0001-6977
Language:: English
Publication year:: 2017
Bibliographic description:: Wnorowski A., Bilek M., Stawarczyk K., Gostkowski M., Olszewski M., Wójciak-Kosior M., Sowa I.: Metabolic activity of tree saps of different origin towards cultured human cells in the light of grade correspondence analysis and multiple regression modeling// ACTA SOCIETATIS BOTANICORUM POLONIAE. -Vol. 86, iss. 2 (2017), s.1-10
DOI:: Digital Object Identifier (open in new tab) 10.5586/asbp.3545
Verified by:: Gdańsk University of Technology