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Abstract
An analytical expression is found allowing the calculation of the secondary emission influenced fluorescence decay of a homogeneous fluorophore solution. Before starting the calculation one has to know the shape of the primary fluorescence decay of the fluorophore and the value of the parameter kappa denoting the ratio of the secondary to primary steady-state fluorescence intensities. The method elaborated by Budo and Ketskemety is recommended for evaluation of the parameter kappa. The main importance of the obtained expression is that it can be used to recover parameters characterizing the fluorescence decay in the absence of secondary emission. The cases of monoexponential, biexponential and multiexponential primary fluorescence decays are discussed in detail.
Authors (3)
-
Hanna Grajek
- Wydział Nauki o Żywności Katedra Fizyki i Biofizyki
-
Ignacy Gryczynski
- Center for Commercialization of Fluorescence Technologies, Health Science Center, University of North Texas .
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Details
- Category:
- Articles
- Type:
- artykuły w czasopismach recenzowanych i innych wydawnictwach ciągłych
- Published in:
-
Methods and Applications in Fluorescence
no. 2,
pages 1 - 12,
ISSN: 2050-6120 - Language:
- English
- Publication year:
- 2014
- Bibliographic description:
- Kuśba J., Grajek H., Gryczynski I.: Secondary emission influenced fluorescence decay of a homogeneous fluorophore solution// Methods and Applications in Fluorescence. -Vol. 2., nr. 1 (2014), s.1-12
- Verified by:
- Gdańsk University of Technology
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